The effect of plant growth regulators on in vitro cucumber (Cucumis sativus L.) regeneration

The main objective of this study was to establish a culture of apical buds, hypocotyls and cotyledons from cucumber (Cucumis sativus L.) to determine their efficiency for regeneration into a whole plant. The genotypes SV0091 and Nomia F1 were used as source for apical buds, hypocotyls and cotyledons as starting explants. The staring explants from both genotypes were cultured on MS medium enriched with different combinations and concentrations of plant growth regulators: MS1 (MS+0.1 mg/l IBA+0.5 mg/l IAA+3.0 mg/l KIN), MS2 (MS+0.5 mg/l IAA+3.0 mg/l BAP), (MS + 0,1 mg/l IBA + 1,5 mg/l BAP + 0,5 mg/l KIN) and MS4 (MS + 0,1 mg/l NAA + 1,0 mg/l BAP). The cultures were grown in a climate chamber under controlled conditions at a temperature of 25 °C and a photoperiod of 16 hours light and 8 hours dark. The de novo plants and shoots in different percentages were obtained from apical buds grown on MS1, MS3 and MS4 media from both genotypes. The hypocotyls and cotyledon segments responded mostly with roots and callus on MS1, MS3 and MS4 media. The MS2 medium proved unsuccessful for micropropagation from all initial explants from both genotypes. After 60-70 days, de novo plants and well-rooted shoots were transferred to plastic pots filled with sterilized peat mixed with perlite and they were put through acclimatization process.