Ivanova, Violeta and Dörnyei, Ágnes and Stefova, Marina and Stafilov, Trajče and Vojnoski, Borimir and Márk, László and Hermosín-Gutiérrez, Isidro and Kilár, Ferenc (2014) Application of a Novel Small-Scale Sample Cleanup Procedure Prior to MALDI-TOF-MS for Rapid Pigment Fingerprinting of Red Wines. Food Analytical Methods, 7 (4). pp. 820-827. ISSN 1936-9751
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Abstract
This study evaluates the anthocyanin and derived
pigment composition of
Vitis vinifera
red wines of Vranec,
Merlot, and Cabernet Sauvignon produced in 2006, 2007, and
2008 vintages from the Tikve
š
wine region in the Republic of
Macedonia.Their profile was established using matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF-MS) technique. A total of 22 anthocyanins
and derived pigments have been identified in the samples including 10 anthocyanins, 1 ethyl-bridged flavanol
–
anthocy-
anin adduct, and 11 pyranoanthocyanins. MALDI-TOF-MS
analysis was performed after solid-phase extraction of the
wines by using, for the first time, the Zip-Tip® C18 stationary
phase, introducing a novel small-scale sample cleanup proce-
dure prior to the rapid MALDI-TOF-MS fingerprinting of
wine samples. 2
′
,4
′
,6
′
-Trihydroxyacetophenone (dissolved in
acetonitrile/water 1:1,
v
/
v
) was used as a matrix. The qualitative
screening of anthocyanins and derived pigments with MALDI-
TOF-MS confirmed the presence of glucoside, acetylglucoside,
and
p
-coumaroylglucoside derivatives of anthocyanins in the
wine samples. Furthermore, pyranoanthocyanins formed by
reactions of anthocyanins with pyruvic acid and acetaldehyde,
as well as flavanol
–
pyranoanthocyanins and ethyl-bridged
flavan-3-ol-anthocyanin adduct pigments have been detected
in the samples.
| Item Type: | Article |
|---|---|
| Subjects: | Agricultural Sciences > Agricultural biotechnology Natural sciences > Chemical sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Violeta Ivanova Petropulos |
| Date Deposited: | 04 Mar 2014 12:10 |
| Last Modified: | 04 Mar 2014 12:10 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/9354 |
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