The Effects of Different Carbon Sources on Biosynthesis of Pecinolytic Enzymes by Aspergillus niger

Mojsov, Kiro (2010) The Effects of Different Carbon Sources on Biosynthesis of Pecinolytic Enzymes by Aspergillus niger. Applied Technologies & Innovations, 3 (3). pp. 23-29. ISSN 1804-1191

Text [5]-Kiro Mojsov, The effects of different carbon sources on biosynthesis of pectinolytic enzymes by Aspergillus niger, ATI, Vol.3, 2010, pp.23-29.pdf Download (165kB)

Abstract

The aim of this work was to investigate the effects of different carbon sources on the nourishing base on the production of pectinolytic enzymes by Aspergillus niger with the aim of optimizing the medium for maximal enzyme production.
Growth and enzymes production by Aspergillus niger were evaluated on glucose, fructose,galactose, xylose, lactose, apple pectin and the dry apple pulp. Results of different carbon sources on base showed maximal endo-pectinolytic activity, endo-PG/328 U L-1 with the pressed apple pulp, compared with endo-PG/140 U L-1 with apple pectin, endo-PG/62 U L-1 with galactose, endo-PG/28 U L-1 with lactose, endo-PG/0.0 U L-1 with glucose and fructose and endo-PG/5.0
U L-1 without carbon source (control).
The growth of the microorganism (dry biomass) on different carbon sources showed maximum dry biomass, 4.5 g L-1 with glucose, compared with dry biomass, 4.3 g L-1 with fructose, 4.0 g L-1 with the pressed apple pulp, 3.5 g L-1 with galactose, 3.0 g L-1 with lactose, 2.2 g L-1 with apple
pectin and 0.8 g L-1 without carbon source (control). Maximal endo-PG production, 328 U L-1 and dry biomass, 4.0 g L-1 by fungus Aspergillus niger was observed in a medium at pH initial, 4.0.
The results presented here will be of commercial importance for using apple pulp as a carbon source for production of pectinolytic enzymes in submerged fermentation.

Item Type:	Article
Subjects:	Engineering and Technology > Industrial Biotechnology
Divisions:	Faculty of Technology
Depositing User:	Kiro Mojsov
Date Deposited:	14 Nov 2012 13:57
Last Modified:	14 Nov 2012 13:57
URI:	https://eprints.ugd.edu.mk/id/eprint/852

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