Assessment of genetic diversity among barley varieties with different origin using simple sequence repeat (SSR) markers

Markova Ruzdik, Natalija and Mihajlov, Ljupco and Ilieva, Verica and Mitrev, Sasa and Arsov, Emilija and Kovacevik, Biljana and Ilievski, Mite and Todevska, Daniela (2024) Assessment of genetic diversity among barley varieties with different origin using simple sequence repeat (SSR) markers. In: IV International Meeting "Agriscience & Practice", APS 2024, 13 June 2024, Faculty of Agriculture, Goce Delcev University, Stip, Republic of North Macedonia.

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Abstract

Barley (Hordeum vulgare L.) is one of the principal cereal crops in the world, because it’s used as raw material in beer production, animal feed and human consumption. By area and production, barley is ranked as the fourth most important variety, followed by wheat, rice and corn. The primary goal in any breeding programme is the grain yield, but the basic requisite it is determine the distinguish among varieties of crop plants and establish their purity. The propose of this research was to determine the genetic diversity among barley genotypes developed in different counties using the SSRs marker. As an experimental material were used twenty-one two row barley varieties with different origin. Three varieties and two promising lines were Macedonian, two varieties were Serbian, two varieties had Croatian origin and the other twelve varieties were developed in Bulgaria. Nineteen SSRs markers were used to characterize the genetic diversity among the barley varieties. Nine of them showed monomorphic bands (Bmag13, GMS1, HVB23D, Bmac0213, Bmac0013, HVM 3, HVM 4, HVM 7 and HVM 9), three markers had high polymorphisms (MGB391, MGB402 and MGB318) and the rest of them didn’t show bands. The calculated polymorphism information content (PIC) values range from 0.163 to 0.574 with an average of 0.380 which shows the importance of the markers for future diversity analysis of barley. Locus MGB318 show the highest PIC and locus MGB391 shows the lowest PIC in this characterized barley genotypes. These results can be useful for barley germplasm management and design of new crosses for future breeding propose.

Key words: barley, simple sequence repeat, genetic diversity, polymorphism information content (PIC), varieties

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Agricultural Sciences > Agricultural biotechnology
Divisions:	Faculty of Agriculture
Depositing User:	Natalija Markova Ruzdik
Date Deposited:	17 Jun 2024 12:40
Last Modified:	17 Jun 2024 12:40
URI:	https://eprints.ugd.edu.mk/id/eprint/34293

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