Physicochemical basis for microbicidal action of disinfectant aqueous solutions

Gjorgjeska, Biljana (2012) Physicochemical basis for microbicidal action of disinfectant aqueous solutions. In: 15th Hellenic Symposium on Medicinal Chemistry, 25-27 May 2012, Athens, Greece.

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Knowing antiseptic activity of chemical disinfectant substances has great practical value. It is evidential that there is the need for defining standard technique for quantitative determination of bactericidal activity of chemical disinfectant substances, as well as the need for defining parameter for comparing various chemical disinfectants. Solution of phenol (5%) was considered as referent standard for evaluation of efficacy of disinfectant aqueous solutions. Phenol coefficient shows how many times bactericidal activity of examined disinfectant is greater or lower than bactericidal activity of standard phenol solution (5%). However, phenol coefficient gives only limited information. Suitability of phenol coefficient for evaluation of nonphenolic disinfectants is still opened question. On the other side the methods for evaluation of antiseptic activity of disinfectant aqueous solutions are microbiological methods.
The aim of this study is to develop a new empirical coefficient which is capable to express the various physic-chemical properties of disinfectant solutions on bactericidal activity. The basic duty of this parameter (Disinfection Activity Coefficient of Solution - DACS) is to express capability for comparison and prediction of disinfectant activity. The DACS index, which is the sum of four terms (fluidity, surface tension, redox potential and osmolality), results in good correlation with the activity at different disinfectant aqueous solutions. The DACS index can be calculated using additive and statistical models. Statistical model is adequate for evaluation of different disinfectant solutions because of better expressing the bactericidal activity then additive model. For analyze of various dilutions of one disinfectant there is no significant difference between this two models. The usefulness of DACS is demonstrated for analyze of bactericidal activities on different disinfectant solutions containing boric acid, chlorhexidne, chlorhexidine with cetrimide, chloroxylenol, chlorophen, eosin, hydrogen peroxide, phenyl mercury borate, povidon-iodine, thiomersal, tosilchloramide and phenol. Results for bactericidal activities obtained from microbiological tests on Staphylococcus aureus was compared with activities predicted with DACS. As the conclusion, it is considered good correlation between experimental and calculated values for bactericidal activity.

Item Type: Conference or Workshop Item (Poster)
Subjects: Agricultural Sciences > Agricultural biotechnology
Agricultural Sciences > Animal and dairy science
Agricultural Sciences > Agriculture, forestry, and fisheries
Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Engineering and Technology > Chemical engineering
Natural sciences > Chemical sciences
Medical and Health Sciences > Clinical medicine
Engineering and Technology > Environmental biotechnology
Engineering and Technology > Environmental engineering
Medical and Health Sciences > Health biotechnology
Medical and Health Sciences > Health sciences
Engineering and Technology > Medical engineering
Agricultural Sciences > Other agricultural sciences
Medical and Health Sciences > Other medical sciences
Natural sciences > Physical sciences
Divisions: Faculty of Medical Science
Depositing User: Biljana Gorgeska
Date Deposited: 03 Dec 2012 13:10
Last Modified: 10 Dec 2012 10:40

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