Pockovska, Marija and Trajkova, Fidanka and Koleva Gudeva, Liljana (2019) Evaluation of androgenic competence of different pepper, tomato and eggplant genotypes. Book of Proceedings / X International Scientific Agriculture Symposium "Agrosym 2019". pp. 525-530. ISSN 978-99976-787-2-0
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Abstract
The methods of biotechnology such as androgenesis introduce new possibilities for faster creation of new varieties or at least faster development of improved genotypes with desirable traits that can give an answer to new abiotic and biotic challenges in agricultural production. Androgenesis is a method that opens possibilities for development of haploids and spontaneous dihaploids plants via anther culture. In vitro anther culture utilized for gaining haploid/dihaploid plants serves as a tool for improvement of some solanaceous crops such as tomato, eggplant and pepper, but it always faces obstacles for high productivity of regenerants in those crops. In the present study, androgenic competence of 3 pepper genotypes (Edita, Bela Duga and Homera), 3 tomato genotypes (Bellfort, Rally and Policarpo) and 1 eggplant genotype (Domaci srednje dugi) were evaluated. The pepper anthers were cultured according to the method developed by Dumas de Valux et al. (1981), tomato anthers were cultivated according to Corral-Martínez et al. (2011), while eggplant anthers were cultivated according to Dumas de Valux and Chambonnet (1982). The experiment showed that androgenesis was successfully implemented only in pepper genotype Edita, whereas the eggplant genotype did not show any response to anther induction media. Cultivation of anthers from all tomato genotypes resulted only in callus formation. Our results are one more confirmation that androgenesis applied on pepper, tomato and eggplant has its limitations and the successfulness of androgenesis depends on many factors as growing conditions and donor plant age, donor plant genotype, microspore developmental stage, culture media and cultivation conditions.
| Item Type: | Article |
|---|---|
| Subjects: | Agricultural Sciences > Agricultural biotechnology Agricultural Sciences > Agriculture, forestry, and fisheries |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Fidanka Trajkova |
| Date Deposited: | 04 Dec 2019 10:05 |
| Last Modified: | 04 Dec 2019 10:05 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/23051 |
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