Smilkov, Katarina and Petreska Ivanovska, Tanja and Petrusevska Tozi, Lidija and Petkovska, Rumenka and Hadzieva, Jasmina and Popovski, Emil and Stafilov, Trajče and Grozdanov, Anita and Mladenovska, Kristina (2013) Optimization of the formulation for preparing Lactobacillus casei loaded whey protein-Ca-alginate microparticles using full-factorial design. Journal of Microencapsulation. ISSN 0265-2048 (print), 1464-5246 (online)
Full text not available from this repository.Abstract
Context: This article presents specific approach for microencapsulation of Lactobacillus casei
using emulsion method followed by additional coating with whey protein.
Methods: Experimental design was employed using polynomial regression model at 2nd level
with three independent variables, concentrations of alginate, whey protein and CaCl2.
Physicochemical, biopharmaceutical and biological properties were investigated.
Results: In 11 series generated, negatively charged microparticles were obtained, with size
6.99–9.88 mm, Ca-content 0.29–0.47mg per 10mg microparticles, and viability of the probiotic
9.30–10.87 log10CFU/g. The viability after 24 hours in simulated gastrointestinal conditions was
between 3.60 and 8.32 log10CFU/g.
Discussion: Optimal formulation of the microparticles that ensures survival of the probiotic
and achieves controlled delivery was determined: 2.5% (w/w) alginate, 3% (w/w) CaCl2 and
3% (w/w) whey protein.
Conclusion: The advantageous properties of the L. casei-loaded microparticles make them
suitable for incorporation in functional food and/or pharmaceutical products.
| Item Type: | Article |
|---|---|
| Subjects: | Natural sciences > Biological sciences Natural sciences > Chemical sciences Medical and Health Sciences > Health biotechnology Medical and Health Sciences > Health sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Katarina Smilkov |
| Date Deposited: | 25 Nov 2013 15:03 |
| Last Modified: | 27 Oct 2014 11:30 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/7144 |
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