Cellular cryobiology - a review of basic concepts and "operating-design" of cryopreserved cells

Balint, Bela and Pavlovic, Mirjana and Abazovic, Dzihan and Toroman, Sanja and Grubovic Rastvorceva, Rada M. and Dinic, Marija and Todorovic-Balint, Milena (2023) Cellular cryobiology - a review of basic concepts and "operating-design" of cryopreserved cells. Vojnosanitetski pregled, 80 (11). pp. 899-905. ISSN 0042-8450

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Abstract

Cryopreservation (CP) is a thermodynamically well-defined operating system, specific for each cell type or “biosubstratum”, that protects cellular organelles, isolated cells, cell clusters, or tissues during cooling to ultra-low temperatures. This method is beneficial when cells are biologically and/or thermally unstable using liquid-state cell preservation.
The increased use of myeloablative treatments (combined with SCs rescue), as well as intensified application of
cell-mediated therapies, has resulted in superior requirements for both the SCs and practical operating procedures to improve cell yield and recovery, as well as minimize cellular damages during harvesting and/or CP.
CP is a well-working system that protects cellular organelles, isolated cells, cell clusters, or tissues during freezing (by ultra-low cooling), long-term cryostorage, and rapid thawing procedures, and it is beneficial in cases where cells are vulnerable and unstable during durable preservation inliquid-state. The major CP requirement is to reduce cell thermal damage (“cryoinjury-score”) and its consequences.
Existing CP techniques applicable in cryo-practice should
be re-evaluated and improved. Further basic research and
(pre)clinical cryo-investigations are recommended or required to define well operating “cryo-biosystem” (specific
for each cell type) in order to obtain an optimized post-thaw cell recovery and viability.

Item Type:	Article
Impact Factor Value:	0,3
Subjects:	Medical and Health Sciences > Clinical medicine
Divisions:	Faculty of Medical Science
Depositing User:	Rada Grubovik Rastvorceva
Date Deposited:	17 Oct 2024 07:55
Last Modified:	17 Oct 2024 07:55
URI:	https://eprints.ugd.edu.mk/id/eprint/34843

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