HPLC- ESI-Q TRAP-MS/MS analysis of flavonoids and nonflavonoids in red wine

Ivanova, Violeta and Biesaga, Magdalena and Bogeva, Elena and Pobozy, Ewa (2024) HPLC- ESI-Q TRAP-MS/MS analysis of flavonoids and nonflavonoids in red wine. In: 22nd International Summer School on Bioanalysis, 7-14 July 2024, Charles University, Faculty of Science, Prague, Czech Republic.

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Abstract

In this study, a high performance liquid chromatography (HPLC) coupled to tandem mass spectroscopy (MS/MS) operated in electrospray ionization (ESI) and quadrupole linear ion trap (Q TRAP) negative mode was applied for simultanous determination of nonflavonoids and flavonoids in Kratošija red wine. A total of 27 phenolic compounds, including 13 nonflavonoids (10 phenolic acids, 2 stilbenes and 1 stilbenoid) and 14 flavonoids (6 flavan-3-ols, 4 flavonols, 2 flavones, 1 flavanone and 1 flavanonol) were identified within 55 minutes by using a Phenomenex Luna C18(2) column (3 µm, 100 Å, 100 x 2 mm), with flow rate at 0.2 mL/min, column temeperature at 30°C and a gradient elution system of 8 mM formc acid in water (eluent A) at pH 2.8 and acetonitrile (eluent B). The relative amounts of phenolic compounds were calculated as areas of the ion extracted peaks of the individual compounds in wine. Gallic acid was the dominant phenolic acid, followed by p-coumaric and syringic acids. Resveratrol-3-glucoside (piceid) was present in highest amount compared to resveratrol and viniferin. From the group of flavonols, myricetin was the dominant component and from flavan-3-ols, procyanidins B1 and B3 dominated in wine. Flavones chrysin and luteolin, flavanone naringenin and flavanonol taxifolin were reported for the first time in Macedonian red wine.

Key words: flavonoids, nonflavonoids, HPLC- ESI-MS/MS, Kratošija red wine.

Acknowledgements
This work was supported by the CEEPUS Network (RO-0010) Teaching and Lerning Bioanalysis covering the study stay of Violeta Ivanova-Petropulos at the Department of Chemistry, University of Warsaw, Poland, where the analyses have been performed.

Item Type:	Conference or Workshop Item (Lecture)
Subjects:	Natural sciences > Chemical sciences Agricultural Sciences > Other agricultural sciences
Divisions:	Faculty of Agriculture
Depositing User:	Violeta Ivanova Petropulos
Date Deposited:	12 Sep 2024 10:19
Last Modified:	12 Sep 2024 10:19
URI:	https://eprints.ugd.edu.mk/id/eprint/34618

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