Determination of antioxidant activity of cold-pressed edible oils from Republic of North Macedonia by Hemoglobin assay

Kostadinovic Velickovska, Sanja and Mitrev, Sasa and Arsov, Emilija and Dimovska, Daniela and Ilieva, Fidanka and Mot, Augustin Catalin and Silaghi-Dumitrescu, Radu and Ilieva, Fidanka (2024) Determination of antioxidant activity of cold-pressed edible oils from Republic of North Macedonia by Hemoglobin assay. In: 4th International Meeting Agriscience & Practice (ASP 2024), 13 June 2024, Stip, Republic of North Macedonia.

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Abstract

In the present study a new approach, based on cell-free hemoglobin (Hb) analysis, is proposed to
evaluate antioxidant activity of cold-pressed edible oils from sunflower, flax and sesame seeds.
The new modified method is based on the spectrophotometric measurement of Hb concentration
at specific wavelength (412 nm). Hemoglobin ascorbate peroxidase activity inhibition (HAPX)
assay was applied for the first time in order to compare the antioxidant activity of cold pressed
edible oils. HAPX assay shows the capability of an extract obtained from an oil to quench the
HbFeIV resulted by hydrogen peroxideinduced damage upon HbFeIII. Transportation of oxygen
via hemoglobin is possible by bonding of an oxygen molecule to iron in the ferrous state (Fe2+).
Oxyhemoglobin produced by side reaction undergoes autoxidation forming methemoglobin
(Fe3+) and in this oxidation state hemoglobin cannot bind any oxygen. In this case Fe3+ from
methemoglobin activates hemoglobin and form ferryl Fe4+. Ferryl hemoglobin is produced in the
body in case of stress, illness and induces peroxidation of lipids (Benedec, Vlase, Oniga, Moţ,
Damian, Hanganu, Duma & Silaghi-Dumitrescu, 2013). Hemoglobin ascorbate peroxidase activity
inhibition (HAPX) assay measure the capability of the extracts from cold-pressed oils to quench
the HbFeIV resulted by hydrogen peroxide-induced damage upon HbFeIII. The results showed
the highest value of the antioxidant potential for extract from flaxseed oil (590.1±3.9 mg Trolox/L
oil). The extract from sunflower oil showed a lower value and the lowest antioxidant potential
measured by this assay was obtained for sesame seed oil (280.2±1.9 mg Trolox/L oil).

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Natural sciences > Chemical sciences
Divisions:	Faculty of Agriculture
Depositing User:	Sanja Kostadinovik
Date Deposited:	29 Jul 2024 07:34
Last Modified:	29 Jul 2024 07:34
URI:	https://eprints.ugd.edu.mk/id/eprint/34467

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