Development of [18F]fluoromisonidazole synthesis method by cartridge purification

Cocevska, Maja and Marija, Atanasova and Katerina, Kolevska and Maja, Velichkovska and Filip, Jolevski and Emilija, Janevik Ivanovska (2023) Development of [18F]fluoromisonidazole synthesis method by cartridge purification. In: 11th Balkan Congress of Nuclear Medicine, 30 May - 02 Jun 2024, Skopje, North Macedonia.

Text (E-Book of abstract) 2024 Balkan_Congress_of_Nuclear_medicine_2024_ABSTRACT_E-BOOK-1.pdf - Published Version Download (14MB)

Abstract

Hypoxic tumors are typically resistant to radiotherapy and chemotherapy and require
alternative treatment strategies. Nitroimidazoles derivatives are an important class of compounds because they are retained in higher levels in hypoxic tumor cells than the
corresponding normoxic cells. [18F]fluoromisonidazole ([18F]FMISO) as nitroimidazole derivative with 18F radioisotope is widely used radiotracer for imaging hypoxia. The direct 18F-fluorination using a tosylate precursor and [18F]F- is a common method for the introduction of fluorine-18 into 1-(2'-nitro-1'-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonylpropanediol precursor (NITTP). Fully automated radiosynthesis procedures of [18F]FMISO by cartridge purification are described only by few authors.
The goal of this work was to take a view of developing of synthesis method of [18F]FMISO by solid phase extraction (SPE) method using IBA Synthera module and to study quality control parameters. Parameters for labeling and hydrolysis were
optimized with regard to temperature, time and the amount of precursor. SCX, Alumina and six different RP extraction cartridge (HLB light, HLB plus LP, C18, tC18, C18 environmental, PS-RP) were used during development of [18F]Fluoromisonidazole synthesis method by cartridge purification. The quality control of [18F]FMISO was performed in accordance with the requirements of European Pharmacopoeia. The cartridges elution samples and waste samples were observed only for chemical by-products and radiochemical purity with HPLC and TLC analysis.
In this study, we successfully synthesized final product with 53,18 ± 3,44 % radiochemical yield, based on in-house prepared reagents with 5 mg NITTP and original cassettes for IBA Synthera. [18F]FMISO complies with requirements of Ph. Eur. Monograph for Fluoromisonidazole (18F) injection.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Maja Cocevska
Date Deposited:	17 Jun 2024 09:45
Last Modified:	17 Jun 2024 09:45
URI:	https://eprints.ugd.edu.mk/id/eprint/34283

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