[18F]Fluoromisonidazole synthesis method:development and optimization by cartridge purification

Cocevska, Maja and Atanasova, Marija and Kolevska, Katerina and Velickovska, Maja and Koziorowski, Jacek and Janevik-Ivanovska, Emilija and Angelovska, Bistra and Ugrinska, Ana (2023) [18F]Fluoromisonidazole synthesis method:development and optimization by cartridge purification. Radiochimica Acta. ISSN 2193-3405

Text Chochevska et al., 2023.pdf - Published Version Download (802kB)

Abstract

18F]Fluoromisonidazole ([18F]FMISO) as nitroimidazole derivative with 18F radioisotope is a widely known and studied hypoxia marker for PET imaging. A number of automated synthesis modules and purification strategies for
production of [18F]FMISO have been described in recent
years. The goal of this work was to develop [18F]FMISO
synthesis process with Synthera module with solid phase
extraction (SPE) Sep-Pak purification cartridges. To adjust
the reaction conditions we synthesized [18F]FMISO under
different reaction conditions and using various reversed phase (RP) purification cartridges (HLB light, HLB plus, tC18, C18 environmental, Chromafix PS-RP). The synthesis was performed by nucleophilic substitution of commercial 1-(2′-nitro-1′-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonylpropanediol precursor and subsequent acidic hydrolysis. Further, the product mixture was purified by passing through the SPE cartridge. The produced [18F]FMISO
was retained on the cartridge, while the impurities passed
through the cartridge into a waste. The retained [18F]FMISO
was then eluted with small amounts of ethanol in water and
eluates were collected in the final product vial. The product sample was subjected to quality control tests, while for waste sample chemical and radiochemical tests were performed.
We have developed an efficient synthesis method of [18F]
FMISO with cartridge purification with good radiochemical
yield (RCY) and high chemical and radiochemical purity
in accordance with the Ph. Eur. Monograph for Fluoromisonidazole (18F) injection.

Keywords: production; purification; purity; Sep-Pak cartridges; [18F]Fluoromisonidazole

Item Type:	Article
Impact Factor Value:	2
Subjects:	Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Maja Cocevska
Date Deposited:	28 Nov 2023 08:41
Last Modified:	02 Jul 2024 07:36
URI:	https://eprints.ugd.edu.mk/id/eprint/32624

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