Influence of collagen induced arthritis on C3 and C4 components on the complement at the white laboratory rat

Spasov, Mire and Gjorgoski, Icko (2022) Influence of collagen induced arthritis on C3 and C4 components on the complement at the white laboratory rat. Knowledge International Journal, 52 (3). ISSN 2545-4439

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Abstract

Rheumatoid arthritis is a special group of diseases in human pathology that manifest clinical, serological, and pathohistological signs of autoreactive activation of lymphocyte clones. Activation of the immune system in these diseases is seen in the occurrence of autoantibodies to one or more autoantigens, which most commonly occurs when the immune system will lost control of lymphocytes that are specifically autoreactive with own organism. The aim of the study was to determine whether type II collagen treatment would cause an effect on the immune system, haematological and biochemical parameters, and among others on C3 and C4 complement components in white laboratory rats of the Wistar strain, both half, on the 30th and 60th day after immunization. Induced rheumatoid arthritis leads to different effects on the individual examined parameters, and also affects the reduction of C3 and C4 components of the complement, but with values that are not in the domain of statistical significance. White laboratory rats of the Wistar breed were used as the experimental model. Healthy animals of both sexes at the age of seven weeks, bred according to keeping standards experimental animals on the laboratory animal farm at the Faculty of Natural Sciences and Mathematics-Skopje. In the experiment collagen-induced arthritis has been shown to elicit a reaction in almost 100% of immunized rats with collagen type-II. They were divided into four groups; control group (n = 20) male animals, control group (n = 20) female animals, experimental group (n = 30) male animals, which were treated with collagen type-II and experimental group (n = 30) female animals who were also treated with type II collagen. Tests were performed on the 30th and 60th day after immunization. The prepared highly purified collagen type-II according to a strictly defined protocol is applied with syringes and small needles in the knee joint of the hind right leg. We took blood for analysis on the 30th and on 60th day after immunization. To determine the C3 and C4 components of complement I we used the method of radial immunodiffusion. Check the results of the analyzes were made on the Minineph device, from the manufacturer The Binding Site from the UK with laser pointing immunoephelometric method. From the studies that were done on the impact of collagen induced arthritis on the C3 and C4 components of complement in men and females, on the 30th and 60th day of treatment it can be concluded that there are reduced values for both parameters in all groups and in both sexes of experimental animals. The resulting results suggest that values for both sexes for both the 30th and 60th day of treatment have reduced values, which are not in the domain of statistical significance. The general conclusion is that animal immunoassays in animal models and drawing a parallel with the clinical picture of rheumatoid arthritis in patients has made a great contribution to clarifying many issues related to the disease.

Item Type:	Article
Subjects:	Medical and Health Sciences > Basic medicine
Divisions:	Faculty of Medical Science
Depositing User:	Mire Spasov
Date Deposited:	07 Jun 2023 12:48
Last Modified:	07 Jun 2023 12:48
URI:	https://eprints.ugd.edu.mk/id/eprint/31866

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