Ivanova, Violeta and Zendelovska, Dragica and Stefova, Marina (2005) HPLC determination of hydrochlorothiazide in urine after solid-phase extraction. Macedonian pharmaceutical bulletin, 51 (1,2). pp. 23-28. ISSN 1409 - 8695
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Abstract
A simple, rapid and precise HPLC method has been developed for the assay of hydrochlorothiazide in urine. The clean-up of the
urine samples was carried out by solid-phase extraction using HLB cartridges. Extraction recovery was 94.00-100.28 %. HPLC separation was performed with isocratic elution on Hypersil BDS C18 column (100 x 4.0 mm I.D., 3 µm particle size) protected with appropriate guard column. The mobile phase was 18 % acetonitrile and 0.025 mol/L solution of KH2
PO4
, pH 4 at flow rate of 0.3 mL/min.
Detection of the substances was performed at 220 nm. The calibration curves were linear in the range of 2-50 µg/mL. The developed
method is validated by checking its accuracy, precision and stability. The detection limit is 2 µg/mL hydrochlorothiazide. The method is
proved to be convenient for routine analysis of hydrochlorothiazide in urine.
| Item Type: | Article |
|---|---|
| Subjects: | Natural sciences > Chemical sciences Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Violeta Ivanova Petropulos |
| Date Deposited: | 05 Nov 2012 08:22 |
| Last Modified: | 28 Oct 2013 12:45 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/316 |
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