Nakov, Dimitar and Andonov, Sreten and Trajchev, Metodija (2016) Antioxidant status in dairy cows during transition period. Journal of Agricultural, Food and Environmental Sciences, 68. pp. 1-8.
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26. ANTIOXIDANT STATUS IN DAIRY COWS DURING TRANSITION PERIOD.pdf Download (270kB) |
Abstract
During the transition period important metabolic changes occur in dairy cows, which can also
experience oxidative stress. The two years prospective study was carried out to assess the changes
occurring in the activity of antioxidant enzymes superoxid dismutase (SOD) and glutathion
peroxidase (GPX) in three physiological periods: dry period 21 days before calving, period from
beginning of lactation until 21st day in lactation, and period from 22nd to 42nd day in lactation.
Determination of the enzyme activity was assayed on blood serum by using spectrofotometric
methods. The average activity of SOD in period before calving was 24.20±2.319 mU/mg proteins,
reaching the value of 43.94±3.864 mU/mg proteins in the second period and in the third period from
22nd to 42nd day in lactation, the activity was 26.77±2.201 mU/mg proteins. The average activity of
GPx in period before calving was 251.79±9.363 mU/mg proteins, than increasing in period from
beginning of lactation until 21st day in lactation (372.45±17.533 mU/mg proteins) and in the third
period from 22nd to 42nd day in lactation, the activity was 319.40±14.307 mU/mg proteins.
Physiological stages in transition period when blood samples was taken, showed statistical significant
influence (p<0.001) on SOD and GPx activity in blood serum. Significant difference in the activity of
SOD in blood serum was found in period before calving as compared to period from calving to 21st
day in lactation, and also in period from calving to 21st day in lactation as compared to period from
22nd to 42nd day in lactation. Significant differences in the activity of GPx in blood serum was found
between all three physiological periods, but biggest significant difference in activity was found in
period before calving as compared to periods in lactation. There were statistically significant positive
correlations between the activity of GPx and SOD in blood serum. Dairy cows experience oxidative
stress in transition period from gestation to lactation.
Key words: dairy cows, oxidative stress, superoxid dismutase, glutathion peroxidase.
| Item Type: | Article |
|---|---|
| Subjects: | Agricultural Sciences > Veterinary science |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Dimitar Nakov |
| Date Deposited: | 30 Aug 2022 09:45 |
| Last Modified: | 30 Aug 2022 09:45 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/30140 |
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