Bosnakovski, Darko and Xu, Zhaohui and Gang, Eun Ji and Galindo, Cristi L and Liu, Mingju and Simsek, Tugba and Garner, Harold R and Agha-Mohammadi, Siamak and Tassin, Alexandra and Coppee, Frederique and Belayew, Alexandra and Perlingeiro, Rita R and Kyba, Michael (2008) An isogenetic myoblast expression screen identifies DUX4-mediated FSHD-associated molecular pathologies. Embo Journal, 27. pp. 2766-2779.
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An isogenetic myoblast expression screen identifies DUX4-mediated FSHD-associated molecular pathologies.pdf Download (1MB) | Preview |
Abstract
Facioscapulohumeral muscular dystrophy (FSHD) is caused by an unusual deletion with neomorphic activity. This deletion derepresses genes in cis; however which candidate gene causes the FSHD phenotype, and through what mechanism, is unknown. We describe a novel genetic tool, inducible cassette exchange, enabling rapid generation of isogenetically modified cells with conditional and variable transgene expression. We compare the effects of expressing variable levels of each FSHD candidate gene on myoblasts. This screen identified only one gene with overt toxicity: DUX4 (double homeobox, chromosome 4), a protein with two homeodomains, each similar in sequence to Pax3 and Pax7. DUX4 expression recapitulates key features of the FSHD molecular phenotype, including repression of MyoD and its target genes, diminished myogenic differentiation, repression of glutathione redox pathway components, and sensitivity to oxidative stress. We further demonstrate competition between DUX4 and Pax3/Pax7: when either Pax3 or Pax7 is expressed at high levels, DUX4 is no longer toxic. We propose a hypothesis for FSHD in which DUX4 expression interferes with Pax7 in satellite cells, and inappropriately regulates Pax targets, including myogenic regulatory factors, during regeneration.
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Darko Bosnakovski |
| Date Deposited: | 30 Nov 2012 14:25 |
| Last Modified: | 12 Feb 2013 11:34 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/2526 |
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