Milev, Mishko and Maksimova, Viktorija and Ruskovska, Tatjana (2020) Determination of genotoxic effect of capsaicin on human peripheral blood mononuclear cells with Comet assay. In: Nutraceuticals in balancing redox status in ageing and age-related diseases, NutRedOx COST Action CA16112, 02-03 March 2020, Belgrade .
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Abstract
Capsaicin is the major representative of the group of proto-alkaloids called capsaicinoids, isolated from hot peppers. Despite the positive health effects of capsaicin seen previously, there are also studies that suggest that capsaicin may act as cytotoxic and genotoxic agent on some human cells.
A considerable number of assays exist for detection of different genotoxic effects of various compounds in experimental systems. The Comet assay is widely used because it is technically simple, sensitive, relatively fast, and cost-effective, and DNA damage can be detected and quantified in different cell types without requirement for cell culture.
The aim of this work was to evaluate the genotoxic potential of capsaicin on human peripheral blood mononuclear cells.
The cells are embedded in agarose and lysed, generating nucleus-like structures in the gel – nucleoids. Following alkaline electrophoresis, the DNA strands migrate towards the anode and the extent of migration correlates with the number of strand breaks (SB) in the nucleoid. The migration is visualized and scored with a light microscope after silver staining.
Thirty minutes of treatment with capsaicin concentrations in the range of 100 - 200 µmol/L resulted in high occurrence of single and double SB. Concentration of 50 µmol/L caused moderate DNA damage, and lower concentrations (20 µmol/L) provoked only minor changes in the genome without DNA lesions.
Although not all types of genotoxic exposures should be expected to result in DNA damage in mononuclear blood cells, the comet assay seems to be a valuable tool for detection of genotoxic exposure in humans.
This work was conducted as part of the NATO project: “A Field Detector for Genotoxicity from CBRN and Explosive Devices” under the Agreement No. SPS G5266 from the Science for peace programme.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Tatjana Ruskovska |
| Date Deposited: | 17 Sep 2020 07:56 |
| Last Modified: | 17 Sep 2020 07:56 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/24465 |
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