Chromatographic techniques for Quality control of freeze dried Radiopharmaceuticals Gel filtration / HPLC

Gel filtration chromatography (referred to as size exclusion chromatography) separates biomolecules based on differences in their molecular size.

The gel media consists of spherical porous particles of carefully controlled pore size through which biomolecules diffuse to different extents based on differences in their molecular sizes. Small molecules diffuse freely into the pores and their movement through the column is retarded, whereas large molecules are unable to enter the pores and are therefore eluted earlier. Hence, molecules are separated in order of decreasing molecular weight, with the largest molecules eluting from the column first.