Tasev, Krste and Stefova, Marina and Ivanova, Violeta (2016) HPLC method validation and application for organic acid analysis in wine after solid-phase extraction. Macedonian Journal of Chemistry and Chemical Engineering, 35 (2). ISSN 1857 - 5552
Preview |
Text
Tasev et al. 2016-organic acids in wine.pdf Download (733kB) | Preview |
Abstract
A solid-phase extraction method followed by reverse phase high-performance liquid chromatography (RP-HPLC) was optimized and validated for the quantitative determination of tartaric, malic, shikimic, lactic, citric and succinic acids in wine. Solid-phase extraction was carried out with C18 cartridges and extraction recoveries for all acids ranging from 98.3 to 103% were obtained. HPLC separation was performed with isocratic elution on a LiChrosorb RP-18 column (250 × 4.6 mm I.D., 5 µm) protected with the appropriate guard column. The mobile phase was a 5 mM solution of H3PO4 with pH 2.1 at a flow rate of 1 ml/min. Detection of the organic acids was performed at 210 nm. The developed method was validated by checking its linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery. The method was applied to the analysis of organic acids in Macedonian red and white wines.
| Item Type: | Article |
|---|---|
| Subjects: | Natural sciences > Chemical sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Violeta Ivanova Petropulos |
| Date Deposited: | 24 Jan 2017 11:14 |
| Last Modified: | 24 Jan 2017 11:14 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/16915 |
Available Versions of this Item
- HPLC method validation and application for organic acid analysis in wine after solid-phase extraction. (deposited 24 Jan 2017 11:14) [Currently Displayed]
Actions (login required)
 |
View Item |