Aleksandrov, Mihail and Maksimova, Viktorija and Gulaboski, Rubin (2016) Development of an electrochemical method for estimation of the antioxidative capacity of syringic and ferulic acid with ABTS as a redox mediator. In: First International Students’ Symposium of Faculty of Medical Sciences, 20 Apr 2016, Stip, Macedonia.
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Abstract
Introduction: Aim of this study was to develop a electrochemical method for investigation of the antioxidative potential of ferulic and syringic acid, by using the antioxidant abilities of Vitamin C, as a referent substance. Ferulic acid is a hydroxycinamic acid found in many traditional herbal medicines. It is commonly found in seeds of plant such as rice, wheat and oats. It has been shown as an effective topical antioxidant which is used as an efficient photoprotective for the skin. It is already known that ferulic acid can be an effective scavenger of free radicals and it has been approved in certain countries as food additive to prevent lipid peroxidation. Additionally, it alleviates oxidative stress as well as decreases glucose levels in diabetic rat. Syringic acid, a naturally occurring O-methylated trihydroxybenzoic acid monomer extracted from Herba dendrobii L., Rosmarinus officinalis L., Origanum vulgare L. and Thymus L. that acts as an antioxidants to clear free radicals. These findings suggest that Syringic acid may act to inhibit the development of diabetic cataract through aldose reductase.
Materials and methods: Standard solutions of syringic and ferulic acid with concentrations of 5 mmol/L, (Sigma Aldrich), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) with concentration of 1 mmol/L and Vitamin C with concentration of 5 mmol/L were used as a standard solutions. Cyclic voltammetry with a standard 5 mL electrochemical cell with three electrodes: glassy carbon electrode, a working electrode, reference electrode (Ag / AgCl), and a counter platinum electrode. The antioxidative properties of these two phenolic acids was performed by using ABTS as a redox mediator. By using Vitamin C as a reference, we evaluated the antioxidative potentials of these two acids. All the experiments were performed in acetic buffer solution (pH=4.6), and KCl (0.001 mol/L) as an electrolyte.
Results: Cyclic voltammograms of these acids have shown an increase of the current of oxidation peaks of the ABTS, which shows that both acids have high antioxidative potential. Ferulic acid in concentration of 0.1 mmol/L gives a rise of the current of the oxidation peak of ABTS (Epa= 0.55 V) for 0.452 µA, and syringic acid (0.1 mmol/L) have shown an increase of 0.827 µA. In comparison, Vitamin C, in the same concentration as phenolic acids, has shown an increase of 0.464 µA. This indicates that antioxidative potential of ferulic acid is approximately the same as the potential of Vitamin C. Syringic acid have shown the highest antioxidative potential from the examined substances.
Conclusions: This simple and fast method for estimation of the antioxidative potential of ferulic and syringic acid, have confirmed their high antioxidative potential. The method can be further developed and improved for estimation of the antioxidative potential of many phytochemicals obtained from the medicinal plants. Moreover this method could serve for investigation of mechanism of oxidation of ferulic and syringic acid, which cannot be predicted by other commonly used spectroscopic method for measuring the antioxidative potential. Therefore, further investigations on the electrochemical properties of these acids are needed.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Uncontrolled Keywords: | antioxidants, cyclic voltammetry, ferulic acid, syringic acid, vitamin C. |
| Subjects: | Natural sciences > Chemical sciences Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Viktorija Maksimova |
| Date Deposited: | 13 Sep 2016 11:27 |
| Last Modified: | 13 Sep 2016 11:27 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/16231 |
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