Maksimova, Viktorija and Gulaboski, Rubin and Koleva Gudeva, Liljana and Ruskovska, Tatjana and Mirceski, Valentin (2016) Comparison of optical and electrochemical methods for determination of the antioxidant effect of some plant metabolites. In: SOE DAAD "From Molecules to Functionalized Materials", 1-5 Sept 2016, Ohrid, Macedonia.
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Abstract
Since plant metabolites became very popular as natural antioxidants, there are many in vitro assays reporting their antioxidant activity. Spectroscopic assays are most frequently used in the literature, but there are few novel electrochemical methods established recently. In this study we compared the antioxidant activity of few phenolic compounds measured by electrochemical method versus their antioxidative potential measured by spectroscopic method, using ascorbic acid as a referent substance. The antioxidative potential of vitamin E, quercetin, capsaicin and dihydrocapsaicin, was examined by means of cyclic voltammetry directly on the surface of a glassy carbon electrode. These results were compared with the results obtained for the same compounds by the spectroscopic FRAP method. It has been shown that the antioxidant potential of these substances obtained by the method of cyclic voltammetry was few times higher compared to their potential measured for the same concentrations by the spectroscopic method, when using the ascorbic acid as reference. It could be suggested that this significant difference is due to the different pH of the medium used in both assays. These results lead to a conclusion that because of some disadvantages of the spectroscopic assays (incubation time and pH of the medium), the electrochemical methods could became more suitable for this purpose. In addition, the antioxidative role of the plant metabolites commonly ingested through nutrition is important in physiological conditions or pH of the medium close to neutral, which are not prescribed in the FRAP protocol, or other spectroscopic methods.
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Subjects: | Natural sciences > Chemical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Rubin Gulaboski |
| Date Deposited: | 12 Sep 2016 12:45 |
| Last Modified: | 12 Sep 2016 13:09 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/16210 |
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