Velickova, Nevenka and Milev, Misko and Petrova, Biljana (2015) Correlation between micronucleus and multinucleated lymphocites in medical personel exposed to radiation. In: XIX Congress of Doctors of Republic of Macedonia, 1-3 Oct 2015, Ohrid, R.Macedonia.
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Abstract
Introduction: Measurement of micronucleus (MN) frequency in peripheral blood lymphocytes (PBL) is extensively used in molecular epidemiology and cytogenetics to evaluate the presence and the extent of chromosomal damage in human populations exposed to genotoxic agents. The observation that chromosome damage can be caused by exposure to ionising radiation can cause major alterations to the genetic material.
Aim of the study: To evaluate the results of lymphocyte micronuclei,and polynuclear lymphicites, necrosis, and apoptosis on the medical personnel occupationally exposed to ionizing radiation.
Matherial and methods: The study included 10 individuals, radiologists who are 20 and ± 5 years ocupationaly exposed to radiation. For evaluation for micronucleus we used micronucleus assay.
Results: In the present study it is evident that an increase in the baseline MN frequency and multinucleated lymphocites. Also we find other cytological changes corresponds to the increase chromosomal damages in the cells.
Coclusion: Cytogenetics provides numerous different biomarkers for evaluating chromosomal instability and scoring of MN in lymphocytes is one that draws a lot of interest The results from the present study support the hypothesis that MN frequency and other cytological changes in lymphocites is a predictive biomarker of cancer risk and chromosomal instability. Detailed evaluation of the genotoxic effects of ionizing radiation needs additional in-depth cytogenetic and molecular investigations.
Key words: micronucleus, lymphocites, radiation, genotoxicology
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Nevenka Velickova |
| Date Deposited: | 22 Jan 2016 11:45 |
| Last Modified: | 22 Jan 2016 11:45 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/14896 |
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