Jansen, Eugene and Ruskovska, Tatjana (2015) Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. International Journal of Molecular Sciences, 16 (11). pp. 27378-27390. ISSN 1422-0067
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Abstract
In this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant
status related to nutrition, which can be used for measurements in large-scale epidemiological
studies. From personal experience, we have come to the following proposal of a set of biomarkers
for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual
antioxidant vitamins E and A, and the carotenoids which can be measured in large series by
HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC.
As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected;
for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay
(biological antioxidant potential). All of these biomarkers can be measured in large quantities by
an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage
conditions, and measurements are discussed. With the selected biomarkers, a good set is presented
for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological
studies. Examples of the successful application of these biomarkers in large international studies
are presented.
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Clinical medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Tatjana Ruskovska |
| Date Deposited: | 10 Dec 2015 10:25 |
| Last Modified: | 10 Dec 2015 10:25 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/14412 |
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