HPLC determination of ogranic acids in Macedonian wines

Ivanova, Violeta and Milanov, Goran and Sarafimovska, Ana and Beleski, Klime and Stefova, Marina and Stafilov, Trajče (2008) HPLC determination of ogranic acids in Macedonian wines. In: 20th Congress of Chemists and Technologysts of Macedonia, 17-20, September, 2008, Ohrid, R. Macedonia.

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Abstract

Quantitative determination of organic acids in wine is important part of fermentation
monitoring during winemaking and can be an additional support for sensorial and microbiological
quality of wines. The levels of organic acids must be carefully controlled to enable proper
fermentation and to prevent spoilage. Organic acids of 21 red, 4 rose and 26 white wines,
harvested 2007, from different wine regions in R. Macedonia, were determined by reverse-phase
high-performance liquid chromatography (RP-HPLC-DAD). Separation of the acids was performed
with C18 column (250 x 4.6 mm, 5 μm) and isocratic elution using aqueous solution of phosphoric
acid, pH 2.2, was applied for separation of the analytes. Determination of acids was made by direct
injection after the appropriate sample dilution and filtration. The highest concentrations of the
tartaric acid were found in Vranec wine, 3.66 mg/L and Chardonnay wine, 3.41 mg/L. Malo-lactic
fermentation was finished for Cabernet Sauvignon and Kadarka wines measuring malic acid
concentrations of 0.021 and 0.020 mg/L, respectively, and highest content of lactic acid was
observed for the same wines as was expected (1.76 and 1.79 mg/L, respectively). The content of
citric acid in the analyzed wines ranged between 81.03 and 986.39 μg/L. Shikimic acid content, as
a parameter for the verification of varietal authenticity of the wines, was determined and the
concentrations were ranged from 15.22 to 36.28 mg/L for red wines and 3.77 to 14.75 mg/L for
white analyzed wines.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Agricultural Sciences > Agricultural biotechnology Natural sciences > Chemical sciences
Divisions:	Faculty of Agriculture
Depositing User:	Violeta Ivanova Petropulos
Date Deposited:	07 Nov 2012 09:17
Last Modified:	28 Oct 2013 13:01
URI:	https://eprints.ugd.edu.mk/id/eprint/563

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