Assessment of genetic diversity among barley varieties with different origin using simple sequence repeat (SSR) markers

Markova Ruzdik, Natalija and Mihajlov, Ljupco and Ilieva, Verica and Mitrev, Sasa and Arsov, Emilija and Kovacevik, Biljana and Ilievski, Mite and Todevska, Daniela (2024) Assessment of genetic diversity among barley varieties with different origin using simple sequence repeat (SSR) markers. Journal of Agriculture and Plant Science, 22 (1). pp. 23-31. ISSN 2545-4455

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Abstract

Barley (Hordeum vulgare L.) is one of the principal cereal crops in the world, because it is used as raw material in beer production, animal feed and human consumption. The propose of this research was to determine the genetic diversity among barley genotypes developed in different counties using the SSR marker. Twenty-one two row barley varieties with different origin were used as an experimental material. Three varieties and two promising lines were Macedonian, two varieties were Serbian, two varieties had Croatian origin and the other twelve varieties were developed in Bulgaria. Eighteen SSR markers were used to characterize the genetic diversity among the barley varieties and two of them had high polymorphisms (MGB402 and MGB318). The calculated polymorphism information content (PIC) values range from 0.163 to 0.574, which shows the importance of the markers for future diversity analysis of barley. Primer MGB318 showed the highest PIC and for MGB391 was obtained the lowest PIC. Using Unweight Pair Group Method, dendrogram tree was constructed and all studied genotypes were divided in three main groups, branched into subgroup. These results can be useful for barley germplasm management and design of new crosses for future breeding propose.

Key words: barley, simple sequence repeat, genetic diversity, polymorphism information content, varieties

Item Type: Article
Subjects: Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Agriculture
Depositing User: Natalija Markova Ruzdik
Date Deposited: 04 Sep 2024 08:21
Last Modified: 04 Sep 2024 08:21
URI: https://eprints.ugd.edu.mk/id/eprint/34597

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