Mastitis makes changes in the blood antioxidant enzyme activity during the transition period of dairy cows: Part II

Nakov, Dimitar and Kuzelov, Aco and Hristov, Slavča and Stankovic, Branislav and Miočinović, Jelena and Cincović, Marko (2024) Mastitis makes changes in the blood antioxidant enzyme activity during the transition period of dairy cows: Part II. In: XIII International Symposium on Agricultural Sciences (AgroReS 2024), 27-30 May, 2024, Trebinje, Bosnia and Herzegovina.

	Text Book_of_Abstracts_AgroReS_2024.pdf Download (10MB)
	Text AgroRes2024_Nakov.pdf Download (1MB)

Abstract

During the transition period in dairy cows, significant metabolic changes occur, potentially leading to oxidative stress. Early postpartum is also the riskiest period due to the increased susceptibility of dairy cows to mastitis. This study aimed to assess the enzymatic antioxidant status by analyzing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in blood obtained from cows with mastitis and comparing it with samples from healthy cows. The two years prospective study was carried out to assess the changes occurring in the activity of antioxidant enzymes SOD and GPX in blood serum in three physiological periods: the dry period 21 days before calving, the period from the beginning of lactation until the 21st day in lactation and period from the 22nd to the 42nd day in lactation. All cows were allocated in groups regarding the season years of calving. Categorisation of the observed population was done following screening for mastitis using udder clinical examination, the California Mastitis Test, and bacteriological culturing. The groups included healthy cows, those with abnormal milk secretion (AMS), and cows with intramammary infection (IMI). Enzyme activity was assessed in blood serum using spectrophotometric methods. The physiological stages in the transition period when blood samples were taken, showed a statistically significant influence (p<0.001) on SOD and GPX activity in blood serum. There were statistically significant positive correlations between the activity of GPX and SOD in blood serum. Health disorders of the mammary gland showed a statistically significant influence on GPX activity in blood serum (p<0.05), but there wasn’t a statistically significant influence on SOD activity in blood serum. The findings suggest that maintaining a balanced diet with sufficient antioxidants and managing environmental stressors can mitigate oxidative stress during the transition period, also reducing the risk of mastitis in early lactation.

Keywords: dairy cows, oxidative stress, udder disorders

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Agricultural Sciences > Animal and dairy science
Divisions:	Faculty of Agriculture
Depositing User:	Dimitar Nakov
Date Deposited:	28 Jun 2024 10:23
Last Modified:	28 Jun 2024 10:23
URI:	https://eprints.ugd.edu.mk/id/eprint/34381

Actions (login required)

View Item