Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/24933
Title: High-risk HPV E6/E7 messenger RNA testing versus HPV DNA testing in women with squamous cell abnormalities of the uterine cervix
Authors: Dabeski, Drage 
Duvlis, Sotirija
Micevska, Megi
Basheska, Neli 
Dabeski, Ana
Antovska, Vesna 
Dimitrov, Goran 
Trajanova, Milka
Krstevska, Iskra
Pavlovski, Borivoje
Keywords: uterine cervix
squamous intraepithelial lesions
squamous cell carcinoma
screening
human papillomavirus
mRNK
DNK
molecular testing
Issue Date: Nov-2017
Publisher: BMJ Publishing Group Ltd, International Gynecologic Cancer Society & European Society of Gynaecological Oncology
Journal: International Journal of Gynecological Cancer
Conference: 20th International Meeting of the European Society of Gynaecological Oncology, 4-7 November, 2017, Vienna, Austria
Abstract: Aims The study is performed in order to compare the results of two HPV screening methods: High-risk HPV E6/E7 mRNA and HPV DNA. Method Comparative prospective study, conducted in the period for April 2016 to March 2017 at the University Clinics for Gynecology and Obstetrics and Radiotherapy and Oncology in Skopje and Institute of Public Health of Republic of Macedonia of 98 sexually active women, age groups of 20 to 60 years, with squamous cell abnormalities on the cervical cytology. In all 98 women were done: HPV DNA testing, High-risk HPV E6/E7 mRNA testing and colposcopy and directed biopsy with endocervical curettage for histopathological analysis. Results Histopathologically, there were: 36.7% non-neoplastic lesions, 20.4% LGSIL cases, 29.6% HGSIL cases and 13.3% invasive squamous cell carcinomas. HPV DNA was found 78.6% of the cases; E6 and E7 transcripts were found in 58.2%. The rates of detection of HPV DNA and E6 and E7 transcripts were 83.3% and 22.2% for cases with non-neoplastic finding; 65.0% and 45.0% for cases with LGSIL; 75.9% and 93.1% for cases with HGSIL and 92.3% and 100% for cases with invasive squamous cell carcinoma. High-risk HPV E6/E7 mRNA testing showed a higher sensitivity than the HPV DNA testing (79.0% and 75.8%), higher specificity (77.8% and 16.7%) and a higher positive predictive value for HGSIL ( 93.1% and 75.9%) and invasive squamous cell carcinoma (100% and 92.3%). Conclusion High-risk HPV E6/E7 mRNA testing could be more powerful than HPV DNA testing for screening and investigation of HGSIL and invasive squamous cell carcinoma.
URI: http://hdl.handle.net/20.500.12188/24933
ISSN: 1048-891X
DOI: http://dx.doi.org/10.1097/01.IGC.0000527296.86225.87
Appears in Collections:Faculty of Medicine: Conference papers

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