Micropropagation of potato seed tubers (Solanum tuberosum L.) under in vitro conditions

Koleva Gudeva, Liljana and Petrova, Irena (2022) Micropropagation of potato seed tubers (Solanum tuberosum L.) under in vitro conditions. Journal of Agriculture and Plant Sciences, 20 (2). pp. 37-43. ISSN 2545-4455

Text JAPS, Vol. 20, No. 2, pp. 37 - 43.pdf Download (1MB)

Abstract

In this paper the results of influence of phytohormone gibberellic acid GA3 on sprout formation in in vivo
conditions and in vitro microtuberization of the potato varieties Agria, Agata, Sunshine, Ultra and Marabel are
presented. The tubers from all varieties utilized in the experiment were certified potato seed tuber material.
The experiment in in vitro conditions was established on sprout explants and nodal segment explants on
the MS medium (Murashige & Skoog, 1962) with addition of different combination and concentration of auxins
and cytokines. Microtuberization was stimulated by rising the concentration of sucrose in MS medium from
40g/l to 60 g/l and 80g/l, respectively.
The in vivo tuber treatment with 30mg/l GA3 was the most effective treatment for all potato varieties in
proliferation of sprouts. All tubers that were treated with GA3 resulted in de novo sprouting of tubers.
The variety Agata resulted with 100% of microtuberization from nodal segment explants on MS medium
supplied with 40g/l and 80g/l sucrose. Microtuberization of the variety Sunshine was stimulated with addition
of 80 g/l sucrose in MS medium.
The developed microtubers were detached from the nodal segments and subcultured on new MS
medium supplied with BAP 4mg/l, KIN 4mg/l and 8% of sucrose to increase their weight.

Item Type:	Article
Subjects:	Agricultural Sciences > Agricultural biotechnology
Divisions:	Faculty of Agriculture
Depositing User:	Liljana Koleva Gudeva
Date Deposited:	16 Feb 2023 08:32
Last Modified:	16 Feb 2023 08:32
URI:	https://eprints.ugd.edu.mk/id/eprint/31400

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