Ivanova, Violeta and Stefova, Marina and Vojnoski, Borimir and Dörnyei, Ágnes and Márk, László and Dimovska, Violeta and Stafilov, Trajče and Kilár, Ferenc (2011) Identification of polyphenolic compounds in red and white grape varieties grown in R. Macedonia and changes of their content during ripening. Food Research International, 44. pp. 2851-2860.
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Abstract
The composition of polyphenols in the skin, seed and pulp extracts of the grapes of Vranec and Smederevka
varieties, and Merlot and Chardonnay as well, was analyzed by liquid chromatography-diode array detectionmass
spectrometry (HPLC-DAD-MS) and MS/MS techniques. Thirty-one phenolic compounds, including
anthocyanins, flavonols and flavan-3-ols, as well as phenolic acids derivatives, have been identified in the
extracts prepared from the grapes at physiological maturity, by mass spectrometry applying electrospray
ionization operated in alternating ion mode and by performing tandem MS experiments in the ion trap mass
analyzer. Grapes were analyzed at three different phases: (i) veraison, (ii) physiological ripeness and (iii) late
harvest, in order to follow the evolution of polyphenolic content during berry development, applying
spectrophotometric methods. Vranec had a higher polyphenolic content compared to Merlot due to the
higher levels of total phenolics and flavonoids in the skins and seeds as well as a higher content of flavan-3-ols
and anthocyanins in the skins, allowing discriminating the varieties. HPLC-DAD-MS analysis presented
significantly higher relative amounts of anthocyanin monoglucosides and p-coumaroylglucosides in Vranec
grapes. Smederevka seeds had a higher amount of flavan-3-ols than Chardonnay, while a higher content of
total phenolics and flavonoids was found in the skins of the Chardonnay variety. Anthocyanin content in both
red varieties increased during the berry ripening, while flavan-3-ols in seeds were mainly accumulated in the
veraison phase followed by decrease of the content with ripening.
| Item Type: | Article |
|---|---|
| Subjects: | Agricultural Sciences > Agricultural biotechnology Natural sciences > Chemical sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Violeta Ivanova Petropulos |
| Date Deposited: | 05 Nov 2012 08:16 |
| Last Modified: | 28 Oct 2013 12:38 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/309 |
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