New evidence for the stolbur phytoplasma development in pepper in Republic of Macedonia

Ivanova, Galina and Mitrev, Sasa and Arsov, Emilija (2017) New evidence for the stolbur phytoplasma development in pepper in Republic of Macedonia. Comptes rendus de l’Acade'mie bulgare des Sciences, 70 (11). pp. 1609-1616. ISSN 2367-5535

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Sweet red pepper and Capsicum annuum var. cerasiforme plants collected from the fields in Eastern Macedonia in the summer period of 2015/16, were proven stolbur positive by performing multilocus genetic analysis, and a comparison with related changes of pigment plastids content in healthy and symptomatic tissue was carried out. PCR amplification on specific tuf , vmp 1 and stamp gene, showed that the identified phytoplasma strains belong to subgroup 16SrXII‑A, since their restriction patterns were indistinguishable from one another and from the patterns characteristic of the STOL (16SrXII‑A) reference strain. For tuf gene, it was possible to identify the HpaII RFLP profiles associated with tuf ‑type b genes. The concentration of chlorophyll a , chlorophyll b , chlorophyll ( a + b ) and carotenoids of non-symptomatic and 100% stolbur-affected pepper leaves were estimated. There was a general trend of decrease of chlorophyll a , chlorophyll b and chlorophyll ( a + b ) concentration, while there was an increase of carotenoids concentration in stolbur-affected pepper plants compared to their non-symptomatic counterparts.
This is the first report of the presence of stolbur phytoplasma on pepper in the Republic of Macedonia.

Item Type: Article
Subjects: Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Agriculture
Depositing User: Emilija Arsov
Date Deposited: 14 Dec 2017 08:54
Last Modified: 14 Dec 2017 08:54

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