Phloem Limited Pathogens of Grapevine in the Republic of Macedonia

Arsov, Emilija and Mitrev, Sasa (2016) Phloem Limited Pathogens of Grapevine in the Republic of Macedonia. LAP LAMBERT Academic Publishing. ISBN 978-3-659-82658-0

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Phytoplasmic changes as well as virus diseases of one of the most promising crops in the Republic of Macedonia, the grapevine, are among the least studied and researched pathogenic changes in the country. In contrast, in other European countries these changes have been investigated very intensively and the results have been presented in relevant publications.
During the period dedicated to the research, the situation was constantly monitored from the aspect of symptomatology. The collected material was analyzed in the Laboratory of plant and environment protection, and typification of the present pathogens was carried out by using modern molecular methods of PCR / RFLP, by studying seven phytoplasmic gene loci: 16S rRNA, tuf, vmp1 gene (stol - 1H0), stamp gene, trxA-truB gene, rplS - csdB gene, cbiQ - glyA gene for the detection of the type of the present phytoplasmas.
By applying serological ELISA technique and RT-PCR molecular diagnostics, we determined the presence of the most widely distributed phloemic viruses in grapevine - Grapevine leafroll associated virus GLRaV (-1, -2, -3, -7).

Item Type: Book
Subjects: Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Agriculture
Depositing User: Emilija Arsov
Date Deposited: 27 Jan 2016 10:58
Last Modified: 23 Dec 2016 13:15

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