Determination of Ferulic Acid with RP-HPLC Method in Oral Solution Containing Extract of Ferula Assafoetida L. and Viscum Album L.

Slavkovska, Jasmina and Shishovska, Maja and Doneva, Dragica and Arsova-Sarafinovska, Zorica and Starkoska, Katerina (2011) Determination of Ferulic Acid with RP-HPLC Method in Oral Solution Containing Extract of Ferula Assafoetida L. and Viscum Album L. In: 5th Congress of Pharmacy of Macedonia with International Participation, 21-25 Sept 2011, Ohrid, Republic of Macedonia.

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Ferula assafoetida L. is a medicinal plant widely used in the traditional medicine. Roots of Asafoetida, produces natural antiviral drug compounds that kill the swine flu virus, H1N1. The aim of this study was to develop and validate a RP-HPLC method for quality control of commercially available oral solution containing extract of Ferula assafoetida L. and Viscum album L. Determination of the content of Ferula assafoetida L. extract in the oral solution was made by determination of ferulic acid, one of components identified in Ferula assafoetida L. plant. The chromatographic separation was achieved on a reversed-phase column Purospher STAR RP -18e (150 x 4.6 mm i.d., particle size 5μm), gradient run (using acetonitrile and 0.01 mol L-1 orthophosphoric acid as a mobile phase), at 25°C temperature. The flow rate was kept at 1.5 ml min-1. Detection of ferulic acid was carried out at 316 nm using photodiode array detector. The proposed method was fully validated according to the ICH guidelines in terms of accuracy, precision, linearity and range. The obtained data for precision, (RSD of 9.69%), and accuracy (recovery of 104.39%) are suitable for this kind of analysis. The linearity of the proposed method was tested in the range of 0.1 – 1.8 μg mL-1 with regression coefficient of 0.9995 obtained. These validation results demonstrate the suitability of the method for quality control of this oral solution with expected content of ferulic acid of about 0.0001%, m/m (1 ppm). This developed HPLC method was proven to be precise, specific, sensitive, and accurate for routine quality assessment of raw material plant Ferula assafoetida L., its extract, and pharmaceutical products.

Item Type: Conference or Workshop Item (Poster)
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 01 Jun 2015 09:43
Last Modified: 01 Jun 2015 09:43

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