Spectrophotometric determination of the content of photosynthetic pigments in some decorative species grown in in vitro and in vivo conditions

Velesanova, Ivana and Trajkova, Fidanka and Koleva Gudeva, Liljana (2019) Spectrophotometric determination of the content of photosynthetic pigments in some decorative species grown in in vitro and in vivo conditions. In: 2nd International Meeting Agriscience & Practice, 12 Apr 2019, Stip.

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Abstract

The photosynthetic activity of species ageratum Ageratum sp., decorative cabbage Brassica oleracea cv. Kyoto red given and carnation Dianthus sp. was determined by examining of the content of chlorophyll a, chlorophyll b, chlorophyll a+b and carotenoids. The content of photosynthetic pigments from in vivo conditions was determined in the sprouting and flowering phenophase. The pigments from in vitro conditions were examined in shoot culture, obtained from meristematic explants, after 30 and 60 days of initial explants placement on MS medium. Extraction of photosynthetic pigments was performed with 96% C2H5OH. Absorbance was determined on UV/VIS spectrophotometer JANWAY 6305 for chlorophyll at 665 nm, chlorophyll b at 649 nm and carotenoids at a wavelength of 470 nm. The content of photosynthetic pigments is lower in in vitro conditions. Generally, in in vivo conditions, the value of photosynthetic pigments is higher at the sprouting phenophase. The average content of chlorophyll a showed that carnations from in vitro conditions had higher value as compared to carnations from in vivo conditions, as an exception from the rule. The flowering of carnation in in vitro conditions was a proof that the photosynthetic activities began earlies in this culture, even in n vitro conditions. The content of chlorophyll b in carnation in in vitro conditions was almost the same as in in vivo conditions. The content of carotenoids in ageratum in in vivo conditions was much higher as compared to the in vitro conditions.

Item Type: Conference or Workshop Item (Poster)
Subjects: Agricultural Sciences > Agricultural biotechnology
Agricultural Sciences > Agriculture, forestry, and fisheries
Divisions: Faculty of Agriculture
Depositing User: Fidanka Trajkova
Date Deposited: 04 Sep 2019 08:16
Last Modified: 04 Sep 2019 08:16
URI: http://eprints.ugd.edu.mk/id/eprint/22397

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