In vitro possibilities for vegetative multiplication of carnation (Dinathus sp.)

Spasenoski, Mirko and Koleva Gudeva, Liljana and Janceva, Milanka (2001) In vitro possibilities for vegetative multiplication of carnation (Dinathus sp.). In: First Symposium on Horticulture, 16-20 Oct 2002, Ohrid, Madedonia.

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For in vitro regeneration of carnation (Dinathus sp.) as initial explants were used apical buds. The explants were cultivated on MS mineral solution, sucrose, agar-agar, inoslitol, casein, Vitamines B1, B6, nicotine acid and phytohormones: 0.5 mg/l IAA and 1 mg/l KIN.
The cultures were kept under controlled conditions. Shoot of apical buds iniciated the hinges multiplication on MS medium with 0.5 mg/l IAA + 1 mg/l KIN + 0.5 mg/l BA. Rooting of shoots were obtained at 2 mg/l IAA + 0.1 mg/l KIN + 2 mg/l adenine. Rooted plants obtained from apical buds after 15-20 days in clime room conditions on a sterile mixture of pert, send and perlit (1:1:1) were will adapted. They normally grew and developed there.

Item Type: Conference or Workshop Item (Paper)
Subjects: Agricultural Sciences > Other agricultural sciences
Divisions: Faculty of Agriculture
Depositing User: Liljana Koleva Gudeva
Date Deposited: 16 Apr 2014 08:11
Last Modified: 16 Apr 2014 08:11

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