The application of molecular-biological techniques upon the determination of the genetic predisposition for diabetes mellitus

Velickova, Nevenka and Paskova, Dobrila (2013) The application of molecular-biological techniques upon the determination of the genetic predisposition for diabetes mellitus. In: The Southeast European Medical Forum, 11-15 Sept 2013, Portoroz, Slovenia.

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Abstract

Diabetes mellitus (DM) is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that goes into bore. It is an autoimmune disease when β cells who secrete insulin, become the subject of a specific attack on its own immune system. Like autoimmune disease is characterized by different long time in patients but at the same time and "quiet" period that does not meet the usual symptoms of the disease. The goal of this work is: assessing of the extent of the population affected by diabetes mellitus; tracing the presence of diabetes mellitus in the general population; tracing the clinical course; indicating the diagnostic procedures for diabetes mellitus; complications in diabetes mellitus; role of laboratory technician and monitoring laboratory analysis; how much this disease is hereditary in the population. Results: Study includes 6000 patients in east part of R. Macedonia, in period of 2005 to 2012. 314 of them are patients with diabetes mellitus and this number has been gradually increasing over the years. As a result of the above analyzes and investigations are coming to the opinion that diabetes mellitus is more and more frequent in general population in Macedonia. Conclusion: In diabetes mellitus secondary occur autoantibodies in a few fractions: glutamic acid decarboxlylase, (GAD), islet cell autoantigen, (ICA), islet cell citoplasmatic autoantigen, (ICCA), islet cell surface autoantigen, (ICSA), insulin autoantibody, (IAA) and tyrosine phosphate (IA-1 I IA-2). Diagnosis of antibodies in serum is sufficient indicator of so-called “prediabetes”. For these reasons the determination of antibodies is particularly useful in the early diagnosis of disease Application of molecular-biological techniques (PCR, Real Time PCR, sequencing and hybridization of DNA) that is proving the gene polymorphism (in HLA) are necessary for early diagnosis on diabetes mellitus (especially tip 1) and genetic predisposition of the patient.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Medical and Health Sciences > Basic medicine
Divisions:	Faculty of Medical Science
Depositing User:	Nevenka Velickova
Date Deposited:	13 Nov 2013 13:37
Last Modified:	13 Nov 2013 13:37
URI:	https://eprints.ugd.edu.mk/id/eprint/8036

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