Evaluation of fluorescent polarization test in diagnosis of human brucellosis

Taleski, Vaso and Stojkoski, S. and Sopovski, Eftim (2006) Evaluation of fluorescent polarization test in diagnosis of human brucellosis. In: Трет конгрес на микробиолозите на Македонија со меѓународно учество, 17-20 May 2006, Ohrid, Macedonia.

	Text EVALUATION OF FLUORESCENT POLARIZATION TEST IN DIAGNOSIS OF HUMAN BRUCELLOSIS.doc Download (25kB)
Preview	Text FPA Poster.pdf Download (364kB) | Preview
	Text FPA 3 kongres na Mikrobiolozi na Makedonija.docx Download (2MB)

Abstract

Introduction: Human brucellosis is a zoonosis that remains a worldwide veterinary, medical, and economical problem. Over 10.000 human cases, approximately 400 cases per year were registered in Macedonia during last 11 years. Different tests for diagnosis of human brucellosis, ranging from culture, serologic tests and numerous PCR-based assays are available.
Mostly used are conventional serologic techniques such as RBT (Rose Bengal, Slide Agglutination Test), Wright (Tube Agglutination Test) and Coombs (Antihuman Globulin Test), than ELISA and competitive-ELISA. Fluorescence Polarization Assay (FPA) developed as an accurate test that can be performed in a field conditions, outside the diagnostic laboratory, has been validated for number of species including cattle, swine, bison.

Objectives: Evaluation of Fluorescence Polarization Assay (FPA) in comparison with ELISA and the Conventional serologic techniques in diagnosis of human brucellosis.

Material and methods: Patient samples were collected at 5 regional hospitals in Macedonia. Many of the patients were on treatment when blood samples were collected. Samples were held frozen at -200C until they were processed. A total of 217 sera samples were tested. Initial diagnoses were made by serology: RBT, Wright and Coombs tests. To detect IgM and IgG anti-brucella antibodies NOVUM Diagnostica micro plates, coated with Brucella LPS antigen and reader ELISA TECAN-Classic, were used.
In FPA, O-polysaccharide prepared from B.abortus strain 1119-3 conjugated with FITC (Fluorescein isothiocyanate) and Fluorescence Polarization Analyzer FPM-1, were used.

Results and conclusions: Sensitivity of Wright, Coombs, ELISA and FPA were 82%, 89%, 98% and 86% respectively. Specificity of Wright, Coombs, ELISA and FPA were 98%, 100%, 100% and 92% respectively.
FPA is a very promising tool in diagnosis of human brucellosis beside diagnosis in animals; further studies concerning sensitivity and specificity are needed. ELISA remains a reference method in serologic diagnosis of human brucellosis.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	Makedonski medicinski pregled, 60:(Suplement 67) str 1-187, Skopje, 2006
Subjects:	Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Vaso Taleski
Date Deposited:	18 Aug 2014 11:40
Last Modified:	18 Aug 2014 11:40
URI:	https://eprints.ugd.edu.mk/id/eprint/5439

Actions (login required)

View Item