Determining coronary microleakage of endodontically treated teeth restored with temporary and permanent restorations

The aim of the study was to determine the coronary microleakage in in-vitro conditions of the endodontically treated teeth, restored with temporary and permanent fillings.
Material: The experimental part of the study included a total of 60 intact single root human teeth, which were endodontically treated in maximum sterile conditions. The intracanal obturation was made with the sealer AH plus and Termafill gutapercha technique. The teeth were divided in 3 groups depending on the coronary restoration: first group – composite resin, second group – Caviton and third group - dental amalgam. Next, we sterilized the teeth with water steam at temperature of 121°С and pressure of 1,5 in autoclave in duration of 15 minutes. After isolating the surface of the roots with two layers of nail polish to the enamel-dentin border, the teeth with the crowns were submerged into bacterial suspension prepared from Proteus mirabilis, Gram- negative motile, rod shaped bacteria, with concentration of the bacterial cells of 107 - 109 in milliliter of solution. They stayed in the bacterial suspension at temperature of 37°С and permanent bacterial concentration for 5 to 30 days. After the time interval of 5 and 30 days, we prepared the teeth for the procedure of histological evaluation of the coronary microleakage and the longitudinal sections were colored by Brow-Bern.
Results: The largest bacterial microleakage after 5 days was determined in the second test group of the teeth restored with the temporary filling material – Caviton (80%). After the period of 30 days, the bacterial microleakage was largest in the second test group and it was 70%.
Conclusion: Coronary restoration as a final procedure in the endodontic therapy should be realized in a period of 5 days, so that the contamination of the endodontic space can be stopped.