MALDI-TOF-MS detection of pigments in wines during maceration

Lelova, Zorica and Stojakovic, Marko and Ivanova, Violeta and Kilár, Ferenc and Lisjak, Klemen and Márk, László (2024) MALDI-TOF-MS detection of pigments in wines during maceration. In: 4th International Meeting Agriscience & Practice (ASP 2024), 13 June 2024, Goce Delcev University, Faculty of Agriculture, Stip, Republic of N. Macedonia.

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Abstract

In this study, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used for qualitative analysis of anthocyanins and derived pigments in red Vranec wines (Vitis vinifera L.) [1, 2]. Wines were produced from whole and crushed berries, and samples were taken after maceration of 3, 5, 7, 9, 10, 12, 15, 18 and 20 days. Extraction of pigments was performed with Sep-PAK® Plus C18 cartridge columns. Sinapic acid was used as a matrix for analyses of the samples. Based on the targeted fragmentation of the ions of interest (their M+ signals), the pigment peaks were identified under positive ion mode. Polyphenolic compounds were detected as flavonoids (anthocyanins and derived pigments, flavonols, flavanones, flavones, isoflavonoids), phenolic acids (hydroxycinnamic acids), lignans and stilbenes. Glucoside, acetylglucoside and p-coumarylglucoside derivatives of anthocyanins were confirmed to be present in the wines.

Keywords: Red wine, polyhenols, anthocyanins, pigments, MALDI-TOF-MS.

References
[1] Ivanova, V., Dörnyei, Á., Stefova, M., Stafilov T., Vojnoski B., Kilár, F., Márk L. (2011). Rapid MALDI-TOF-MS detection of anthocyanins in wine and grape using different matrices. Food Analytical Methods, 4, 108–115. https://doi.org/10.1007/s12161-010-9143-7
[2] Ivanova-Petropulos, V., Dörnyei, A., Stefova, M., Stafilov, T., Vojnoski, B., Márk, L., Hermosín-Gutiérrez, I., Kilár, F. (2014). Application of a novel small-scale sample cleanup procedure prior to MALDI-TOF-MS for rapid pigment fingerprinting of red wines. Food Analytical Methods, 7(4):820-827 DOI: 10.1007/s12161-013-9687-4

Acknowledgments: This work was financially supported by the Central European Exchange Program for University Studies (CEEPUS) Program realized through the CEEPUS network (Teaching and Learning Bioanalysis) covering the study stay of Zorica Lelova at the University of Pécs, Hungary where the analysis were performed.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Natural sciences > Chemical sciences Agricultural Sciences > Other agricultural sciences
Divisions:	Faculty of Agriculture
Depositing User:	Violeta Ivanova Petropulos
Date Deposited:	12 Sep 2024 10:21
Last Modified:	12 Sep 2024 10:21
URI:	https://eprints.ugd.edu.mk/id/eprint/34621

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