64Cu-complex of the bifunctional chelator with PSMA targeting molecule in ex-vivo biodistribution study in healthy rats

Cocevska, Maja and Tripunoski, Toni and Nikolovski, Saso and Jolevski, Filip and Dimitrova Sumkovska, Jasmina and Ugrinska, Ana (2021) 64Cu-complex of the bifunctional chelator with PSMA targeting molecule in ex-vivo biodistribution study in healthy rats. Journal of the Macedonian Medical Association, 75 (3). ISSN 0025-1097

Text MMP202175- 64CU-COMPLEX OF THE BIFUNCTIONAL CHELATOR WITH PSMA.pdf Download (3MB)

Abstract

Тhe development of new diagnostic radioactive biochemical products and effective therapeutic radiopharmaceuticals is very important in the era of a personalized approach in the management of cancer patients.
Consistent expression of prostate specific membrane
antigen (PSMA) in metastatic prostate cancer helps us
in finding the relevant radioactive prostate-specific
membrane antibody. The radiopharmaceuticals used in
molecular imaging require a simple radiolabeling (radiosynthesis) procedure, good target-to-organ sensitivity, and metabolic stability.
The aim of this study was to label bifunctional chelator, DOTA, covalently bound to a PSMA targeting vector (DOTAGA-(I-y)fk(Sub-KuE)) with Copper-64 (64Cu)
and to examine its physicochemical properties and pharmacokinetic characteristics. Healthy adult rats of Wistar strain were used to carry out experiments through ex
vivo biodistribution with intravenous radiopharmaceutical
administration of (7±1.5) MBq. Target organs and tissues
were harvested 60 min post-injection.
Also, in vitro radiochemical stability of radiolabeled
molecule 64Cu-DOTA-PSMA was studied with TLC
method, 24 h post preparation of the product.
The obtained data showed a low uptake (≤0.45 % IA g-1)
in the majority of organs, except for the liver (1.21±
0.48 % IA g-1) and the kidneys (6.10±0.98 % IA g-1),
indicating them as clearance organs.
The in vitro stability study confirmed a relatively high
stability and the product can be safely used during that
period.

Item Type:	Article
Subjects:	Medical and Health Sciences > Basic medicine
Divisions:	Faculty of Medical Science
Depositing User:	Maja Cocevska
Date Deposited:	27 Jun 2024 10:48
Last Modified:	27 Jun 2024 10:48
URI:	https://eprints.ugd.edu.mk/id/eprint/34341

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