Duvlis, Sotirija and Dabeski, Drage (2024) Association of HPV E6/E7 mRNA Expression with IL-10 c. -592C>A Single Nucleotide Polymorphism. In: EUROGIN 2024 - International Multidisciplinary HPV Congress, 01-12 Apr 2022, Dusseldorf, Germany.
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Abstract
Background/Objectives: High-risk HPV persistent infections with HPV E6/E7 mRNA expression are a highly predictive
marker for the progression of cervical intraepithelial lesion toward cervical cancer (CCa). Still, only a low percentage of
HPV-positive women, the infection remains persistent with continuous HPV E6/E7 mRNA expression. The individual host's
immune response is an important factor influencing viral biological activity and HPV E6/E7 mRNA expression.
Immunomodulatory effects of cytokines such as IL-10 and its genetic variants may confer variation in host response toward
HPV clearance as well as the influence of the expression of these viral oncogenes.
Methods: This study evaluates the association of IL-10 c. -592C>A single nucleotide polymorphism (SNP) with HPV E6/E7
mRNA positivity. We conducted a case-control study that included 159 HPV DNA-positive women and 113 HPV
DNA-negative women with no prior history of HPV positivity or cervical abnormality, as a control group. Women from both
groups were genotyped for IL-10 c. -592C> A using RFLP analysis. Additionally, the cases group was tested for HPV E6/E7
mRNA expression using commercial tests. The statistical significance of the association was assessed by the chi-square test and
the OD ratio.
Results: The results showed the CC genotype (59.3%) was significantly more common in women positive for both tests
compared to those positive only for HPV DNA (39.2%) [p = 0.018; OR = 2.25 (95% CI: 1.14-4.45)], and compared to control
and HPV DNA positive groups together [p = 0.04; OR: 1.68 (95% CI: 1.03-2.75)], but not compared to the control group alone
(49.6%). The frequency of the C allele in the HPV E6/E7 mRNA positive group (78.2%) was significantly more frequent [p =
0.016, OR= 1.88 (95% CI: 1.11-3.16)] than only HPV DNA positive (65.7%) and with borderline significance compared to
both HPV E6/E7 mRNA negative groups [p = 0.04, OR=1.88 (95% CI: 1.11-3.16)].
Conclusions: In conclusion, the C/CC variant of IL-10 c. -592C>A SNP may influence a more frequent rate of HPV E6/E7
mRNA expression after the onset of HPV infection. This genotype is not associated with susceptibility to this infection given
the absence of association with HPV DNA alone. These results should be confirmed in a larger epidemiological study
involving a much larger number of women
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Sotirija Duvlis |
| Date Deposited: | 27 May 2024 08:22 |
| Last Modified: | 23 Aug 2024 10:52 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/34145 |
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