Resolving a Consecutive Two-Step Electrochemical Transformation of Water-Soluble Redox Enzymes with Inverted Potentials-A Theory of Diffusional EEC’ Mechanism in Square-Wave Voltammetry

Gulaboski, Rubin (2024) Resolving a Consecutive Two-Step Electrochemical Transformation of Water-Soluble Redox Enzymes with Inverted Potentials-A Theory of Diffusional EEC’ Mechanism in Square-Wave Voltammetry. [Experiment] (Unpublished)

Text (MATHCAD Simulation Protocol) Enzyme Systems with Inverted Redox Potentials in Diffusional EEC'- 2024 Gulaboski-MATHCAD.pdf - Draft Version Download (826kB)

Abstract

Consecutive electron transfer steps involve a series of sequential redox reactions where electrons are successively transferred to a specific redox-active center within a protein structure. One of the key functions of consecutive electron transfer steps in water-soluble proteins is in energy metabolism and electron transport chains. For systems with inverted potentials, voltammetry gives a single peak in which both electron transfers are hidden. In this model, we show that the diffusional EEC’ mechanism provides an elegant approach to distinguish the two-step electrochemical transformation of enzymes with inverted potential. This can be achieved by changing the rate of the catalytic (regenerative) reaction only.

Item Type:	Experiment
Subjects:	Natural sciences > Chemical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Rubin Gulaboski
Date Deposited:	15 Apr 2024 07:25
Last Modified:	15 Apr 2024 07:25
URI:	https://eprints.ugd.edu.mk/id/eprint/34022

Actions (login required)

View Item