Analytical approaches for assessing immunoconjugates integrity and characterization prior to radiolabeling

Apostolova, Paulina and Arev, Marija and Janevik-Ivanovska, Emilija (2023) Analytical approaches for assessing immunoconjugates integrity and characterization prior to radiolabeling. In: Interdisciplinary School In Nuclear Medicine - ISI NucMed, 3–7 July 2023, Nantes, France.

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Abstract

Immunoconjugates, which have highly specific targeting towards certain antigens, are molecules that are built from three parts: an antibody, a therapeutic payload, and a linker between them.
Based on the mechanism of the therapeutic agent, they are divided into defined groups: antibody-drug conjugates (therapeutic payload), radioimmunoconjugates (radioisotope), or immunotoxins (catalytic, protein toxins).
The major interest of this research is focused on immunoconjugates ready to be radiolabeled, and the analytical techniques used for assessing their integrity and characterization.
Testing of the immunoconjugates generally can be as quality control testing in qualified good manufacturing practices laboratories or characterization using research and development methods.
The used techniques, electrophoresis (reducing conditions), Infrared spectroscopy and Raman spectroscopy, give information about the integrity, primary structure, and the number of chelators attached to the mAb with MALDI – TOF analysis. This can be as primary analysis for integrity and characterization of immunoconjugates prior radiolabeling.
The success of a radiolabeling process, as well as the distribution of immunoconjugate after labeling to tumor cells, depends primarily on antibody characteristics, the appropriate chelator, and the method of their conjugation.
The choice of methods used for quality control of the formed immunoconjugates is crucial to correctly assess and perform categorization prior to radiolabeling process.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Medical and Health Sciences > Basic medicine
Divisions:	Faculty of Medical Science
Depositing User:	Marija Arev
Date Deposited:	24 Aug 2023 11:13
Last Modified:	24 Aug 2023 11:13
URI:	https://eprints.ugd.edu.mk/id/eprint/32089

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