Effect of collagen induced arthritis on the CRP in white laboratory rat

Spasov, Mire and Gjorgoski, Icko and Spasova, Verica (2021) Effect of collagen induced arthritis on the CRP in white laboratory rat. Knowledge International Journal, 47 (4). ISSN 2545-4439

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Abstract

: Arthritis is an insidious and devastating disease of the joints with consequences on other organs and organ systems. In rheumatoid arthritis, whether it is induced or appears spontaneously, and especially in chronic form, in animals and humans there are changes such as inflammation, swelling, pain, difficulty moving, but also changes in the blood. Our study aimed to determine whether treatment with type II collagen would have an effect on the immune system, haematological and biochemical parameters and, among other things, on the C-reactive protein in white laboratory rats of the Wistar strain, male and female in the 30th and the 60th day after post-immunization. In addition, it was shown that induced rheumatoid arthritis leads to different effects on the individual parameters examined and overall it is seen that it has an impact on the change of C-reactive protein. Collagen-induced arthritis caused a reaction in almost 100% of immunized rats with collagen type-II. As an experimental model were used white laboratory rats of the Wistar breed, healthy animals of both sexes at seven weeks of age, bred according to experimental animal standards. They were divided into four groups; control group (n = 20) male animals, control group (n = 20) female animals, experimental group (n = 30) male animals treated with collagen type-II and experimental group (n = 30) female animals which also thus, were treated with type II collagen. Tests were performed on the 30th and 60th day after immunization. The prepared and highly purified collagen type-II, according to a strictly defined protocol is applied with syringes and small needles in the knee joint of the right hind leg. Blood was taken for analysis on the 30th and 60th day after post-immunization. Compared values for the control male and female groups give almost identical results for the value of C-reactive protein. On the 30th day the arthritic male group in comparison to the arthritic female group shows different dynamics of results. Thus, in the male group there is an insignificant decrease in the value of C-reactive protein, while in the female group there is an insignificant increase in the value. If we compare the results on the 60th day of immunization, it’s noticed that in the male group there is a tendency to further increase in value, while in the female group it tends to slightly decrease. The stimulating effect of rheumatoid arthritis on C-reactive protein on day 60 in the female group of arthritic animals, compared with controls, is not as pronounced as in male experimental animals. The results show changes in all groups of animals, regardless of sex. The values in the treated animals change compared to the control group, but they are without statistical significance. It’s generally concluded that animal autoimmunity in animal models and making parallels in the clinical picture of rheumatoid arthritis in patients make a major contribution to clarifying many disease-related questions.

Item Type:	Article
Subjects:	Medical and Health Sciences > Basic medicine
Divisions:	Faculty of Medical Science
Depositing User:	Mire Spasov
Date Deposited:	07 Jun 2023 12:46
Last Modified:	07 Jun 2023 12:46
URI:	https://eprints.ugd.edu.mk/id/eprint/31863

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