Ivanova, Violeta and Zendelovska, Dragica and Stefova, Marina and Stafilov, Trajče (2008) HPLC method for determination of verapamil in human plasma after solid-phase extraction. Journal of Biochemical and Biophysical Methods, 70. pp. 1297-1303.
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Abstract
A simple, rapid and precise HPLC method has been developed for the assay of verapamil in human plasma. The clean up of the plasma samples was
tested using several adsorbents for solid-phase extraction and best recovery was obtained using mixed-mode cartridges (HLB - hydrophilic-lipophilic
balance) ranging between 94.70 and 103.71%. HPLC separation was performed with isocratic elution on Lichrospher 60 RP-select B column (250 mm ×
4mmI.D., 5 μmparticle size). The mobile phasewas 40%acetonitrile and 0.025mol/LKH2PO4 with pH 2.5 at flow rate of 1 mL/min.Diltiazemwas used as
internal standard and the detection wavelength was 200 nm. The calibration curves were linear in the range of 10–500 ng/mL. The developed method is
convenient for routine analysis of verapamil in human plasma.
| Item Type: | Article |
|---|---|
| Subjects: | Natural sciences > Chemical sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Violeta Ivanova Petropulos |
| Date Deposited: | 05 Nov 2012 08:20 |
| Last Modified: | 28 Oct 2013 12:44 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/313 |
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