Applicability of a Spectrophotometric Method for Assessment of Oxidative Stress in Poultry

Regenhard, Petra and Nakov, Dimitar and Sauerwein, Helga (2014) Applicability of a Spectrophotometric Method for Assessment of Oxidative Stress in Poultry. Macedonian Veterinary Review, 37 (1). pp. 43-47. ISSN 1857-7415

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Abstract

Applicability of a Spectrophotometric Method for Assessment of Oxidative Stress in Poultry Petra Regenhard Institute of Animal Science, Physiology and Hygiene Group, University of Bonn, Katzenburgweg 7-9, D-53115 Bonn, Germany Dimitar Nakov University Ss. Cyril and Methodius in Skopje, Faculty of Agricultural Sciences and Food, Institute for Animal Biotechnology, Department for Animal Health and Welfare Blvd. Aleksandar Makedonski, 1000 Skopje, Republic of Macedonia Helga Sauerwein Institute of Animal Science, Physiology and Hygiene Group, University of Bonn, Katzenburgweg 7-9, D-53115 Bonn, Germany Abstract

Oxidative stress is discussed as being related to health, welfare, and also to product quality. Recently, a spectrophotometric method based on the Fenton reaction (reactive oxygen metabolites, ROM) has been proposed to characterize pro-oxidative stressors in body fluids by determining early products of oxidation from proteins, lipids and nucleic acids. The applicability of this method for blood samples from various bird species was tested. The validity of the method for mammalian domestic animals was demonstrated by linearity yielded with increasing amounts of serum. In contrast, serum obtained from chicken yielded ROM concentrations below the limit of detection when serum volumes comparable to mammalian samples were used. Applying higher amounts of serum was not increasing the optical density readings in a linear way. To assess whether the lack of sensitivity and linearity is a problem limited to chicken or poultry in general, we tested sera from laying hens, turkey, duck, goose, pheasant, pigeon, and guinea fowl. For most of the species tested, the method could not be validly applied. There is no reason to assume that oxidative stress in birds may not exist, therefore we conclude that bird specific matrix effects hinder the valid application of the ROMs test in poultry.
3 1 2014 11 18 2015 43 47 10.14432/j.macvetrev.2013.10.002 http://creativecommons.org/licenses/by-nc-nd/4.0 10.14432/j.macvetrev.2013.10.002 https://www.sciendo.com/article/10.14432/j.macvetrev.2013.10.002 https://www.sciendo.com/article/10.14432/j.macvetrev.2013.10.002 https://content.sciendo.com/view/journals/macvetrev/37/1/article-p43.xml

Item Type:	Article
Subjects:	Agricultural Sciences > Animal and dairy science Agricultural Sciences > Veterinary science
Divisions:	Faculty of Agriculture
Depositing User:	Dimitar Nakov
Date Deposited:	30 Jan 2023 10:10
Last Modified:	30 Jan 2023 10:10
URI:	https://eprints.ugd.edu.mk/id/eprint/30984

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