Distinction between film loss and enzyme inactivation in protein-film voltammetry: a theoretical study in cyclic staircase voltammetry

Gulaboski, Rubin (2022) Distinction between film loss and enzyme inactivation in protein-film voltammetry: a theoretical study in cyclic staircase voltammetry. Monatshefte für Chemie - Chemical Monthly, 153. pp. 1-10. ISSN 0026-9247

Text Enzyme Inhibition and Film Loss in Protein-FIlm Voltammetry-Monatshefte-Gulaboski oct 2022.pdf - Published Version Restricted to Registered users only until 1 November 2024. Download (1MB) | Request a copy

Abstract

Inactivation of redox enzymes and the loss of enzyme’s material from its adsorbed film on electrode surface are events that have similar outputs when studied under conditions of protein-film voltammetry. In the current work, we present a series of novel theoretical results under conditions of protein-film cyclic staircase voltammetry that clearly reveal how to distinguish between these two events. Theoretical model elaborated in this work comprises a surface electrode mechanism in which the initial form of redox enzyme is involved in same time in an electrode transformation and in an irreversible reaction of deactivation. While a large set of theoretical voltammetric curves simulated at different conditions are discussed, we also give a tabular overview of some characteristic cyclic voltammetric patterns of this electrode mechanism. The simulated voltam�mograms can help the experimentalists to distinguish the phenomenon of loss of protein film from the electrode mechanism associated with enzyme deactivation. In addition, we give hints to apply suitable methodologies to get access to the kinetics of both steps involved in this electrochemical mechanism.

Item Type:	Article
Impact Factor Value:	1.613
Subjects:	Medical and Health Sciences > Basic medicine Natural sciences > Chemical sciences Natural sciences > Physical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Rubin Gulaboski
Date Deposited:	01 Nov 2022 08:48
Last Modified:	01 Nov 2022 08:48
URI:	https://eprints.ugd.edu.mk/id/eprint/30388

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