Polymerase chain re- action test for detection of SARS-CoV-2 virus and variants

Pesnacka, Aneta and Boshevska, Golubinka and Velickova, Nevenka and Jancevska, Elizabeta and Vukovik, Maja and Buzarova, Teodora and Nikolovska, Gordana and Presova, Ardijan (2022) Polymerase chain re- action test for detection of SARS-CoV-2 virus and variants. Archives of Public Health, 14 (1). pp. 1-11. ISSN 1857-7148 (online)

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Abstract

The first human cases of coronavirus disease (COVID-19), a disease caused by the new coronavirus, were first reported on December 31, 2019 in China. Тhe virus was named temporary as 2019 new coronavirus (2019 novel coronavirus-2019 -nCoV), or finally as SARS-CoV-2.
The aim of this paper is to give an overview of the laboratory detection of SARS-CoV-2 virus with Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR) in real time, as well as detection of viral mutations.
The samples used in the study were taken from all geographical regions in RNM, including the 10 regional Centers for Public Health throughout the country, hospital patients from COVID19 hospitals across the country and from the COVID19 checkpoint at the Institute of Public Health. All samples were tested using RT-PCR in real time. Additional assays have been applied for the identification of the circulating variants.
The continuous surveillance of the variants of concern (VOC),as well as the newly emerged variants can allow for the public health officials to modify their approach to disease control and management and intervene more effectively as well as in a timely manner in order to prevent major morbidity and mortality from COVID-19.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Divisions: Faculty of Medical Science
Depositing User: Nevenka Velickova
Date Deposited: 25 Jan 2022 08:07
Last Modified: 23 Aug 2023 09:31
URI: https://eprints.ugd.edu.mk/id/eprint/29028

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