Detection of placental chromosomal aberrations in early spontaneous abortions in correlation with the histologic findings

Kubelka-Sabit, Katerina and Bozinovski, Gorgi and Jasar, Dzengis and Filipovski, Vanja and Lazarevski, Slobodan and Ivanovski, Mitko and Plaseska-Karanfilska, Dijana (2017) Detection of placental chromosomal aberrations in early spontaneous abortions in correlation with the histologic findings. Macedonian Medical Review, 71 (1). pp. 64-71. ISSN 0025-1097

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Abstract

Using a variety of molecular techniques, it has been established that loss of pregnancy occurs in one to two thirds of all fertilized embryosin the first trimester. In about 50% of the cases, chromosomal abnormalities are the cause of early spontaneous abortion. Several histological characteristicsof the placenta, such as presence of villous stromal cavitations, fetal erythro-cytes, umbilical cord, fetal tissue,etc. are suggested as predictive factors for aneuploidy.Two hundred and thirty one cases were analyzed in this prospective study, 50 cases were control artificial abortionsand 181 cases were early spontaneous abor-tionsanalyzed in the period from May 2012 to De-cember 2014. Standard histopathological analysis and molecular techniques based on polymerase chain reaction were usedto analyze the samples.Usingmolecular techniques, aneuploidy was detected in 53.1% of the samples. The most frequently detected aneuploidy was trisomy 16, followed by trisomy 22, 21, 14 and 18. The molecular analysis also enabled dis-tinctionof maternal and paternal origin of the alleles.In the histopathological sample analysis, binary logis-tic regression analysis indicated the presence of trophob-lasticproliferation (p=0.008) and the absence of fetal red bloodcells (p=0.001) as independent significant fac-tors in the prediction of aneuploidy in early sponta-neous abortion.In conclusion, our results show that clinically relevant andaccurate diagnosis of early spontaneous abortionwhich candetermine its causecan only be achieved bya controlled process of selection of the material,histo-pathological and molecular analysis, followed bya necessary correlation of these results.

Item Type:	Article
Impact Factor Value:	0.877
Uncontrolled Keywords:	aneuploidy, histopathological analysis,miscarriage, molecular analysis
Subjects:	Medical and Health Sciences > Basic medicine Medical and Health Sciences > Clinical medicine Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Vanja Filipovski
Date Deposited:	02 Dec 2021 17:13
Last Modified:	02 Dec 2021 17:13
URI:	https://eprints.ugd.edu.mk/id/eprint/28673

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