Rapid detection of mismatch repair proteins by immunohistochemistry in colorectal cancer patients

Kubelka-Sabit, Katerina and Filipovski, Vanja and Dimova, Biljana and Jasar, Dzengis (2019) Rapid detection of mismatch repair proteins by immunohistochemistry in colorectal cancer patients. Virchows Archiv, 475. S281-S281. ISSN 1432-2307 (electronic version) Journal no. 428

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Abstract

Background & Objectives:Lynch syndrome is an inherited disorder thatincreases the risk of many types of cancer, particularly colorectal andendometrial cancer. Therefore, all newly diagnosed colorectal cancersshould be screened for Lynch syndrome. New immunohistochemistry(IHC) based tests which detect mismatch repair (MMR) proteins offerquick and reliable identification ofpatients with probable Lynchsyndrome.Methods:In this prospective study, we evaluated 50 cases of colorectalcancer patients using the Ventana MMR IHC Panel. The panel containsfive primary mouse or rabbit monoclonal antibodies: MLH-1 (M1),PMS2 (EPR3947), MSH2 (G219-1129), MSH6 (44) and BRAF V600E(VE1). The analysis was performed on automated platform VentanaBench Mark GX, using 4μthin tissue sections from representative tu-mour tissue paraffin blocks.Results:Of the 50 analysed cases, 3 cases showed absence of positivityfor MLH1 and PMS2 markers. Two of these cases had MLH1 promoterhypermethylation and were classified as sporadic cancers. One case wasnegative for PMS2 marker and one case was negative for MSH6 marker.In total, 3 of the 50 cases analysed were sent for further Lynch syndrometesting. Two of these three patients were female and also had a history ofendometrial cancer prior to the diagnosis of colorectal cancer. One casewas positive for BRAF V600E antibody.Conclusion:Immunohistochemical detection of MMR proteins enablesquick detection of patient with probable Lynch syndrome. Further iden-tification of the syndrome in patients and family members may result inearly detection and possible cancer prevention in these patients.

Item Type:	Article
Impact Factor Value:	4.064
Uncontrolled Keywords:	missmatch repair proteins, immunohistochemistry, colorectal patients
Subjects:	Medical and Health Sciences > Basic medicine Medical and Health Sciences > Clinical medicine Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Vanja Filipovski
Date Deposited:	06 Dec 2021 08:21
Last Modified:	06 Dec 2021 08:21
URI:	https://eprints.ugd.edu.mk/id/eprint/28669

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