Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application

Brboric, Jasmina and Klisic, Aleksandra and Kotur-Stevuljevic, Jelena and Delogu, Giovanna and Gjorgieva Ackova, Darinka and Kostic, Kristina and Dettori, Maria Antonietta and Fabbri, Davide and Carta, Paola and Saso, Luciano (2021) Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application. Archives of Medical Sciences. ISSN 1734-1922 (In Press)

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Phenols are a large family of natural and synthetic compounds with known antioxidant activity. The
aim of this study was to preform an in vitro screening of natural and natural-like phenol monomers
and their C2-symetric dimers (hydroxylated biphenyls) in order to identify those representatives
which pharmacophores have the strongest antioxidant and the lowest prooxidant activity.
Material and methods
Antioxidative properties of 36 compounds (monomers and their C2-symmetric dimers) were
evaluated in vitro. Different (red/ox) assays were used to measure their total oxidative potential
(TOP), their total antioxidative capacity (TAC), the pro-oxidative-antioxidant balance (PAB) and total
SH-group content (SHG) in a biologically relevant environment. The Pro-oxidative Score,
Antioxidative Score and the Oxy Score were also calculated. Trolox, a water soluble analogue of α-
tocopherol was used as a positive control.
In an assay consisting of pooled human serum 6 of the 36 compounds indicated significant
antioxidant activity (compounds 6, 7, 12, 13, 26, and 27) whereas 4 indicated extremely weak
antioxidant activity (compounds 2, 29, 30, and 31). Within the 36 compounds comprising of
zingerone, dehydrozingerone, aurone, chalcone, magnolol derivatives, in both monomeric and
dimeric forms, the 2 compounds that indicated the highest antioxidant activity were
dehydrozingerone derivatives (compounds 6 and 12). Trolox’s activity was found between the strong
and weak antioxidant compounds analysed in our study.
In this study selected dehydrozingerones were identified as good candidates for in-depth testing of
their biological behaviour and for possible precursors for the synthesis of novel polyphenolic
molecules with potential therapeutic applications.

Item Type: Article
Impact Factor Value: 2.807
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Darinka Gorgieva Ackova
Date Deposited: 19 May 2021 08:26
Last Modified: 19 May 2021 08:26

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